Anti-inflammatory activity of arctigenin against PCV2 infection in a mouse model.

Abstract

Arctigenin (ACT) is a novel anti-inflammatory lignan extracted from Arctium lappa L, a herb commonly used in traditional Chinese herbal medicine. In this study, we investigated the molecular mechanism whereby ACT inhibits PCV2 infection-induced proinflammatory cytokine production in vitro and in vivo. We observed that in PCV2 infection+ACT treated PK-15 cells, proinflammatory cytokine production was significantly reduced, compared to the PCV2-infected cells. The transfection and luciferase reporter assay confirmed that ACT suppressed NF-κB signalling pathway activation following PCV2 infection in PK-15 cells. Furthermore, western blotting demonstrated that ACT suppressed the NF-κB signal pathway in PCV2 infection-stimulated PK-15 cells by inhibiting the translocation of p65 from the cytoplasm to the nucleus and IκBα phosphorylation. BALB/c mice were used as a model to evaluate the anti-inflammatory effect of ACT in vivo. We found that the BALB/c mice inoculated with PCV2 infection + ACT treated showed a significant reduction of proinflammatory cytokine production in serum, lung and spleen tissue, compared to the PCV2-infected mice. Western blotting confirmed that ACT suppressed the NF-κB signal pathway in PCV2-infected mice by inhibiting the translocation of p65 from the cytoplasm to the nucleus and IκBα phosphorylation in lung tissue. Our studies first demonstrate that ACT inhibits PCV2 infection-induced proinflammatory cytokine production by suppressing the phosphorylation and nuclear translocation of NF-κB in vitro and in vivo. These results will help further develop ACT as a Traditional Chinese herbal medicine remedy in the treatment of porcine circovirus-associated diseases.

• Authors: Lijun Wu, Jie Chen, Danna Zhou, Runshan Chen, Xiabing Chen, Zhiyong Shao, Wenhai Yang, Bin He
• Journal: Veterinary medicine and science (Vet Med Sci) Vol. 8 Issue 2 Pg. 700-709 (03 2022) ISSN: 2053-1095 [Electronic] England
• PMID: 34914190 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2021 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd.

Chemical References

• Anti-Inflammatory Agents
• Cytokines
• Drugs, Chinese Herbal
• Furans
• Lignans
• NF-kappa B
• NF-KappaB Inhibitor alpha
• arctigenin

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Circoviridae Infections (drug therapy)
• Cytokines (metabolism)
• Drugs, Chinese Herbal (pharmacology)
• Furans (pharmacology)
• Lignans (pharmacology)
• Mice
• Mice, Inbred BALB C
• NF-KappaB Inhibitor alpha (metabolism)
• NF-kappa B (metabolism)
• Swine