Adenomyoepithelioma (
AME) of the breast is a rare
tumor that is composed of proliferating epithelial and myoepithelial cells. The pathogenesis of
AME remains unclear, and no
breast cancer cells have been identified in such
tumor tissues. In this study, we established patient-derived
breast cancer organoids from the surgical
tumor samples of an elderly Chinese woman with an
AME of the breast. Our findings confirmed the successful establishment of organoids from an
AME of the breast of this patient. A short tandem repeat analysis revealed that the
DNA signature of the
AME of the breast organoids matched the
DNA signature of the original
tumor specimen. Moreover, diameter assay confirmed that the organoids from the breast
AME showed sensitivity to
paclitaxel and
doxorubicin treatments, which was similar to, but lesser than that of primary culture cells. In conclusion, we established an efficient 3-dimensional
breast cancer organoid culture platform from an
AME of the breast. This platform can be effectively used for exploring clinicopathological and genomic characteristics of
AME of the breast to identify possible treatments and increase awareness about this disease entity.