Abstract | OBJECTIVE: MATERIALS AND METHODS: To establish a CRPS animal model, 10-week-old Sprague-Dawley rats were used in the experiment. On the fourth week post tibial fracture surgery, we performed the von Frey test to measure mechanical allodynia. After performing behavioural tests, we collected blood and tissue samples after sacrificing the animals. Enzyme-linked immunosorbent assay and western blot were also performed. RESULTS: The experimental tibia fracture model-induced CRPS animals elicited increased 5-HT3 receptor expression, and the 5-HT transporter was decreased in the brain stem after 4 weeks of surgical intervention. Additionally, in CRPS-induced animals, both the concentration of substance P and the level of interleukin 6 were increased peripherally and centrally. Treatment with the 5-HT3 receptor antagonist, ramosetron, exerted an analgesic effect in the paw withdrawal test and was dependent on the attenuation of the 5-HT3 receptor population with inflammatory pain mediators. CONCLUSIONS: These data suggest that treatment with the 5-HT3 receptor antagonist, ramosetron, in experimental CRPS animal models alleviated pain-related behaviours and may be a new therapeutic option or potential therapeutic agent for patients with CRPS.
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Authors | J H Park, C-H Lee, H D Ham, E-S Choi, C Lee, S Lee |
Journal | European review for medical and pharmacological sciences
(Eur Rev Med Pharmacol Sci)
Vol. 25
Issue 22
Pg. 7051-7057
(Nov 2021)
ISSN: 2284-0729 [Electronic] Italy |
PMID | 34859869
(Publication Type: Journal Article)
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Chemical References |
- Analgesics
- Benzimidazoles
- Cytokines
- Receptors, Serotonin, 5-HT3
- Serotonin 5-HT3 Receptor Antagonists
- Substance P
- ramosetron
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Topics |
- Analgesics
(therapeutic use)
- Animals
- Benzimidazoles
(therapeutic use)
- Brain Stem
(drug effects, metabolism)
- Complex Regional Pain Syndromes
(drug therapy, etiology)
- Cytokines
(metabolism)
- Disease Models, Animal
- Pain
(drug therapy, etiology)
- Rats, Sprague-Dawley
- Receptors, Serotonin, 5-HT3
(metabolism)
- Serotonin 5-HT3 Receptor Antagonists
(therapeutic use)
- Substance P
(metabolism)
- Tibial Fractures
(complications, drug therapy)
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