Abstract | OBJECTIVE: MATERIALS AND METHODS: In this experimental study, 48 adult NMRI male mice were randomly divided into 4 groups (n=12 in each) including Methadone, Methadone with sesame oil, Methadone with CoQ10 and saline. The injections of methadone, saline and sesame oil were performed intraperitoneally for 20 days. 24 hours after last injection, half of the animals in each group (n=6) were randomly assessed for evaluating of spatial memory by radial maze. Following behavioral study, animals were sacrificed, and their brains were removed to evaluate pyknotic cells through histological assessment. The remaining were used to study the expression of Arc, Bax, Bcl-2 and Bdnf genes. RESULTS: Results of the present study showed that daily administration of methadone increased the number of pyknotic neurons in the CA1 hippocampus and altered the expression of Bax, Bdnf, Arc and Bcl-2. However, it did not alter spatial memory comparing to saline group. CoQ10 treatment significantly reduced the number of pyknotic cells and expression of Bax, Bdnf, Arc when compared to the vehicle group treated by sesame oil. However, the expression of Bcl-2 significantly increased as a result of CoQ10 treatment. CONCLUSION:
CoQ10 reduced the neuronal damage caused by methadone in the hippocampus CA1.
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Authors | Maryam Vaselbehagh, Mehdi Sadegh, Hadi Karami, Saied Babaie, Mohammad Hassan Sakhaie |
Journal | Cell journal
(Cell J)
Vol. 23
Issue 5
Pg. 538-543
(Oct 2021)
ISSN: 2228-5806 [Print] Iran |
PMID | 34837681
(Publication Type: Journal Article)
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