Abstract | BACKGROUND: METHODS: Different concentrations of Ampelopsin (0, 10, 25, 50, and 100 μM) were used to treat 786-O cells. Cell viability was detected by MTT assay, colony formation assay, and flow cytometry assay. Transwell assay and Wound healing assay were used to detect cell migration and invasion. Western blot analysis was applied to detect protein expression. RESULTS:
Ampelopsin inhibited cell proliferation and induced apoptosis in RCC. And Ampelopsin can inhibit cell migration and invasion in RCC. All these results changed in a dose-dependent manner. Ampelopsin (100 uM) had the strongest inhibitory effect on cell viability and metastasis. In addition, Ampelopsin negatively regulated the PI3K/AKT signaling pathway in RCC cells. Moreover, Ampelopsin was only cytotoxic to RCC cells. CONCLUSION:
Ampelopsin inhibits cell viability and metastasis in RCC by negatively regulating the PI3K/AKT signaling pathway.
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Authors | Zhonghe Zhao, Yan Jiang, Zhongguo Liu, Qingyan Li, Tiantian Gao, Shengxia Zhang |
Journal | Evidence-based complementary and alternative medicine : eCAM
(Evid Based Complement Alternat Med)
Vol. 2021
Pg. 4650566
( 2021)
ISSN: 1741-427X [Print] United States |
PMID | 34804180
(Publication Type: Journal Article)
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Copyright | Copyright © 2021 Zhonghe Zhao et al. |