Abstract |
The efficient isolation of immune cells with high purity and low cell damage is important for immunotherapy and remains highly challenging. We herein report a cell capture DNA network containing polyvalent multimodules for the specific isolation and in situ incubation of T lymphocytes (T-cells). Two ultralong DNA chains synthesized by an enzymatic amplification process were rationally designed to include functional multimodules as cell anchors and immune adjuvants. Mutually complementary sequences facilitated the formation of a DNA network and encapsulation of T-cells, as well as offering cutting sites of a restriction enzyme for the responsive release of T-cells and immune adjuvants. The purity of captured tumor-infiltrating T-cells reached 98%, and the viability of T-cells maintained ∼90%. The T-cells-containing DNA network was further administrated to a tumor lesion for localized immunotherapy. Our work provides a robust nanobiotechnology for efficient isolation of immune cells and other biological particles.
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Authors | Chi Yao, Chenxu Zhu, Jianpu Tang, Junhan Ou, Rui Zhang, Dayong Yang |
Journal | Journal of the American Chemical Society
(J Am Chem Soc)
Vol. 143
Issue 46
Pg. 19330-19340
(11 24 2021)
ISSN: 1520-5126 [Electronic] United States |
PMID | 34780151
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Animals
- DNA
(immunology)
- Immunotherapy
- Melanoma
(immunology)
- Mice
- Mice, Inbred C57BL
- T-Lymphocytes
(immunology)
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