Serpin peptidase inhibitor, clade E nexin group 1 promotes cellular proliferative capacities and malignant behaviors in glioblastoma through upregulating hairy and enhancer of split-1.

Abstract

PURPOSE:

Glioblastoma (GBM) remains one of the most fatal malignancy with limited available treatment. Serpin peptidase inhibitor, clade E nexin group 1 (SERPINE1) was found up-regulated in multiple cancers and play crucial roles in facilitating tumor progression and metastasis respectively. However, the role of SERPINE1 in glioblastoma was poorly understood.

METHODS:

We tested the hypothesis that SERPINE1 mediated malignant behaviors in GBM via regulating hairy and enhancer of split-1 (HES1).

RESULTS:

First, SERPINE1 is confirmed to be up-regulated in GBM, while further functional analysis demonstrated that SERPINE1 promoted cell proliferation, migration and invasion in GBM by performing the CCK-8 assay, colony formation assay, wound healing assay and transwell assay. Finally, it was proved that SERPINE1 achieved its pro-tumor functions in GBM via regulating the expression of HES1.

CONCLUSIONS:

Collectively, our results highlight the critical contribution of SERPINE1 in a series of malignant characteristics of GBM via regulating the expression of HES1, which shed new light on a new direction to develop a more effective therapeutic management of malignant tumors like GBM.

• Authors: Tao Ma, Kai Qian, Tuoye Xu, Xi Wang, Tengda Qian, Jing Shi, Lixin Li
• Journal: Journal of B.U.ON. : official journal of the Balkan Union of Oncology (J BUON) 2021 Sep-Oct Vol. 26 Issue 5 Pg. 2074-2083 ISSN: 2241-6293 [Electronic] Cyprus
• PMID: 34761619 (Publication Type: Journal Article)

Chemical References

• Plasminogen Activator Inhibitor 1
• SERPINE1 protein, human
• Transcription Factor HES-1
• HES1 protein, human

Topics

• Cell Proliferation
• Glioblastoma (pathology)
• Humans
• Plasminogen Activator Inhibitor 1 (physiology)
• Transcription Factor HES-1 (physiology)
• Tumor Cells, Cultured
• Up-Regulation