Abstract |
PSMA3, a member of the proteasome subunit, has been shown to play a major player in protein degradation. Reportedly, PSMA3 functions as a negative regulator in various cancers including colon, pancreatic and gastric cancers. However, the contributions of PSMA3 to the progression of esophageal squamous cell carcinoma (ESCC) and the underlying mechanism remain unclear. Therefore, in this study, we investigated whether PSMA3 is involved in ESCC progression and the potential underlying mechanism. The results revealed that PSMA3 was highly expressed in the ESCC tumor tissues and functioned as a negative indicator according to the data from The Cancer Genome Atlas (TCGA)/Gene Expression Omnibus (GEO) datasets and clinical patients' samples. Pathway enrichment analysis showed that PSMA3 was closely correlated with ESCC cancer stemness and the inflammatory response; however, this correlation was absent after knockdown of PSMA3 in vitro. We further demonstrated that PSMA3 suppressed CD8+ T-cells infiltration depending on the C-C motif chemokine ligand 3 (CCL3)/C-C motif chemokine receptor 5 (CCR5) axis. Collectively, these results demonstrate the role of PSMA3 in ESCC cancer stemness and the negative regulation of CD8 T-cells infiltration mediated by PSMA3. The results of this study may provide a potential target for the immuno-oncology effect of PSMA3 in ESCC therapy.
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Authors | Jinyan Liu, Jingwen Shao, Chaoqi Zhang, Guohui Qin, Jiayin Liu, Miaomiao Li, Peng Wu, Xuan Zhao, Yi Zhang |
Journal | European journal of immunology
(Eur J Immunol)
Vol. 52
Issue 2
Pg. 338-351
(02 2022)
ISSN: 1521-4141 [Electronic] Germany |
PMID | 34755333
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2021 Wiley-VCH GmbH. |
Chemical References |
- Neoplasm Proteins
- PSMA3 protein, human
- Proteasome Endopeptidase Complex
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Topics |
- Cell Line, Tumor
- Databases, Nucleic Acid
- Esophageal Neoplasms
(enzymology, genetics, immunology)
- Esophageal Squamous Cell Carcinoma
(enzymology, genetics, immunology)
- Gene Expression Regulation, Enzymologic
(immunology)
- Gene Expression Regulation, Neoplastic
(immunology)
- Humans
- Neoplasm Proteins
(biosynthesis, genetics, immunology)
- Proteasome Endopeptidase Complex
(biosynthesis, genetics, immunology)
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