Cholangiocarcinoma is the second most common malignant
tumor in the hepatobiliary system. Compared with data on
hepatocellular carcinoma, fewer public data and prognostic-related studies on
cholangiocarcinoma are available, and effective prognostic prediction methods for
cholangiocarcinoma are lacking. In recent years, ferroptosis has become an important subject of
tumor research. Some studies have indicated that ferroptosis plays an important role in hepatobiliary
cancers. However, the prediction of the prognostic effect of ferroptosis in patients with
cholangiocarcinoma has not been reported. In addition, many reports have described the ability of
photodynamic therapy (
PDT), a potential
therapy for
cholangiocarcinoma, to regulate ferroptosis by generating
reactive oxygen species (ROS). By constructing ferroptosis scores, the prognoses of patients with
cholangiocarcinoma can be effectively predicted, and potential gene targets can be discovered to further enhance the efficacy of
PDT. In this study, gene expression profiles and clinical information (TCGA, E-MTAB-6389, and GSE107943) of patients with
cholangiocarcinoma were collected and divided into training sets and validation sets. Then, a model of the ferroptosis gene signature was constructed using least absolute shrinkage and selection operator (LASSO)-penalized Cox regression analysis. Furthermore, through the analysis of
RNA-seq data after
PDT treatment of
cholangiocarcinoma,
PDT-sensitive genes were obtained and verified by immunohistochemistry staining and Western blot. The results of this study provide new insight for predicting the prognosis of
cholangiocarcinoma and screening target genes that enhance the efficacy of
PDT.