Intra-Articular Injection of miR-29a-3p of BMSCs Promotes Cartilage Self-Repairing and Alleviates Pain in the Rat Osteoarthritis.

Abstract

BACKGROUND:

Stem cells intra-articular injection stagey indicated a potential therapeutic effect on improving the pathological progress of osteoarthritis (OA). However, the long-term effect of stem cells intra-articular injection on the cartilage regeneration remains unclear. miR-29a-3p is predicted to be a critical target for inhibiting insulin-like growth factor-1 expression and may aggravate the progression of OA.

METHODS:

In this study, we investigated the therapeutic efficacy of intra-articular injection of bone marrow mesenchymal stem cells (BMSCs) transfected with miR-29a-3p inhibitor in OA.

RESULTS:

miR-29a-3p inhibitor transfection did not influence cell viability of BMSCs, while the chondrogenic differentiation potential of BMSCs was significantly improved. Interestingly, intra-articular injection of BMSCs with miR-29a-3p inhibition significantly prevented articular cartilage degeneration by up-regulating the expression of Sox 9, Col-2a1, aggrecan and down-regulating the expression of matrix metalloproteinase, as well as relieved pain in OA.

CONCLUSION:

The double effects on cartilage protection and pain relief indicated a great potential of intra-articular injection of miR-29a-3p inhibitor-transfected BMSCs for the treatment of OA.

• Authors: Qing Wang, Yong Chen, Xiaofeng Shen, Ji Chen, Yuwei Li
• Journal: Tissue engineering and regenerative medicine (Tissue Eng Regen Med) Vol. 18 Issue 6 Pg. 1045-1055 (12 2021) ISSN: 2212-5469 [Electronic] Korea (South)
• PMID: 34542842 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2021. The Korean Tissue Engineering and Regenerative Medicine Society.

Chemical References

• MicroRNAs

Topics

• Animals
• Cartilage, Articular
• Injections, Intra-Articular
• Mesenchymal Stem Cells (metabolism)
• MicroRNAs (genetics, metabolism)
• Osteoarthritis (genetics, therapy)
• Pain (metabolism)
• Rats