Liver fibrosis is a patho-physiological process which can develop into
cirrhosis, and hepatic
carcinoma without intervention. Our study extensively investigated the mechanisms of
lncRNA NEAT1 and miR-139-5p in regulating
liver fibrosis progression. Our results demonstrated that the expression of
lncRNA NEAT1 was increased and the expression of miR-139-5p was decreased in fibrotic liver tissues.
LncRNA NEAT1 could sponge miR-139-5p and promoted hepatic stellate cells (HSCs) activation by directly inhibiting the expression of miR-139-5p. The co-localization of
lncRNA NEAT1 with miR-139-5p was shown in the cytosols of activated HSCs. miR-139-5p upregulation could suppress the expression of β-
catenin. The overexpression of β-
catenin promoted HSCs activation. Moreover, we found that β-
catenin could interact with SOX9 promoted HSCs activation. Our further studies demonstrated that SOX9 could bind with the TGF-β1 promoter and promoted the transcription activity of TGF-β1. The upregulation of TGF-β1 further promoted HSCs activation. In vivo study also suggested that
lncRNA NEAT1 knockdown and miR-139-5p overexpression alleviated murine
liver fibrosis.
LncRNA NEAT1 exacerbated
liver fibrosis by suppressing the expression of miR-139-5p. Collectively, our study suggested that miR-139-5p sponged by
lncRNA NEAT1 regulated
liver fibrosis via targeting β-
catenin/SOX9/TGF-β1 Pathway.