Circulating microRNAs as novel diagnostic biomarkers and prognostic predictors for septic patients.

Abstract

This study was to find out novel miRNAs whether could be used as diagnostic or prognostic biomarkers in sepsis. We used miRNAs microarray assays and further confirmed the levels of miRNAs in 151 septic patients' plasma. 56 miRNAs were up-regulated and 74 miRNAs down-regulated in septic patients compared with the healthy volunteers. But only miR-519c-5p and miR-3622b-3p were up-regulated in both septic and septic shock patients. The levels of miR-519c-5p and miR-3622b-3p were statistically higher in 151 septic patients than healthy controls on day 1. The AUC for miR-519c-5p was 0.79 (95% CI, 0.688-0.892, p = 0.001) in the diagnosis of sepsis, and the AUC for miR-3622b-3p 0.752 (95% CI, 0.622-0.881, p = 0.003). The AUC for the combination of these two miRNAs was 0.831 (95% CI, 0.74-0.923, p < 0.001). Besides, the AUC for miR-519c-5p was 0.597 (p = 0.043) in predicting 28-day mortality. MiR-519c-5p, miR-3622b-3p were novel biomarkers for diagnosing septic patients. High miR-519c-5p levels suggest a worse short-term prognosis. CLINICAL TRIAL REGISTRATION INFORMATION: Name of the registry: Diagnostic and prognostic value of circulating miRNA in patients with sepsis; Trial registration ID: ChiCTR-DDD-17013150; registered 30 October 2017; http://www.chictr.org.cn/edit.aspx?pid=22528&htm=4.

• Authors: Jiyou Yao, Ka Yin Lui, Xiaoguang Hu, Enhe Liu, Tingting Zhang, Li Tong, Jinghong Xu, Fa Huang, Yanping Zhu, Minqiang Lu, Changjie Cai
• Journal: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases (Infect Genet Evol) Vol. 95 Pg. 105082 (11 2021) ISSN: 1567-7257 [Electronic] Netherlands
• PMID: 34520874 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2021 Elsevier B.V. All rights reserved.

Chemical References

• Biomarkers
• Circulating MicroRNA

Topics

• Adult
• Aged
• Aged, 80 and over
• Biomarkers (blood)
• China
• Circulating MicroRNA (blood)
• Diagnostic Tests, Routine (methods)
• Female
• Humans
• Male
• Middle Aged
• Prognosis
• Sepsis (diagnosis)
• Young Adult