Nonimmunogenic Hydrogel-Mediated Delivery of Antibiotics Outperforms Clinically Used Formulations in Mitigating Wound Infections.

Abstract

Treatment of chronic wound infections caused by Gram-positive bacteria such as Staphylococcus aureus is highly challenging due to the low efficacy of existing formulations, thereby leading to drug resistance. Herein, we present the synthesis of a nonimmunogenic cholic acid-glycine-glycine conjugate (A6) that self-assembles into a supramolecular viscoelastic hydrogel (A6 gel) suitable for topical applications. The A6 hydrogel can entrap different antibiotics with high efficacy without compromising its viscoelastic behavior. Activities against different bacterial species using a disc diffusion assay demonstrated the antimicrobial effect of the ciprofloxacin-loaded A6 hydrogel (CPF-Gel). Immune profiling and gene expression studies after the application of the A6 gel to mice confirmed its nonimmunogenic nature to host tissues. We further demonstrated that topical application of CPF-Gel clears S. aureus-mediated wound infections more effectively than clinically used formulations. Therefore, cholic acid-derived hydrogels are an efficacious matrix for topical delivery of antibiotics and should be explored further.

• Authors: Sandeep Kumar, Sanjay Pal, Jyoti Thakur, Parul Rani, Kajal Rana, Animesh Kar, Raunak Kar, Devashish Mehta, Somesh Kumar Jha, Manas Kumar Pradhan, Dolly Jain, Kajal Rajput, Sarita Mishra, Munia Ganguli, Aasheesh Srivastava, Ujjaini Dasgupta, Veena S Patil, Avinash Bajaj
• Journal: ACS applied materials & interfaces (ACS Appl Mater Interfaces) Vol. 13 Issue 37 Pg. 44041-44053 (Sep 22 2021) ISSN: 1944-8252 [Electronic] United States
• PMID: 34491724 (Publication Type: Journal Article)

Chemical References

• Anti-Bacterial Agents
• Cholic Acids
• Dipeptides
• Drug Carriers
• Hydrogels
• Ciprofloxacin

Topics

• Animals
• Anti-Bacterial Agents (chemistry, therapeutic use)
• Cholic Acids (chemical synthesis, chemistry)
• Ciprofloxacin (chemistry, therapeutic use)
• Dipeptides (chemical synthesis, chemistry)
• Drug Carriers (chemical synthesis, chemistry)
• Drug Liberation
• Hydrogels (chemical synthesis, chemistry)
• Male
• Mice, Inbred BALB C
• Mice, Inbred C57BL
• Staphylococcal Skin Infections (drug therapy)
• Staphylococcus aureus (drug effects)
• Wound Infection (drug therapy)
• Mice