Betanin Prevents Experimental Abdominal Aortic Aneurysm Progression by Modulating the TLR4/NF-κB and Nrf2/HO-1 Pathways.

Abstract

Betanin, a bioactive ingredient mostly isolated from beetroots, exhibits a protective effect against cardiovascular diseases. However, its effects on abdominal aortic aneurysm (AAA) have not been elucidated. In this study, an AAA model was constructed by infusion of porcine pancreatic elastase in C57BL/6 mice. Mice were then administered with betanin or saline intragastrically once daily for 14 d. Our results showed that treatment with betanin remarkably limited AAA enlargement and mitigated the infiltration of inflammatory cells in the adventitia. The increased expression of proinflammatory cytokines and matrix metalloproteinases (MMPs) was also significantly alleviated following betanin treatment. Furthermore, betanin suppressed the activation of toll-like receptor 4 (TLR4)/nuclear factor-kappaB (NF-κB) signaling in the aortic wall, and downregulated the levels of tissue-reactive oxygen species as well as circulating 8-isoprostane by stimulating the nuclear factor-E2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway. Taken together, these data suggest that betanin may attenuate AAA progression and may be used as a therapeutic drug against AAA.

• Authors: Renfeng Qiu, Shuxiao Chen, Fang Hua, Shuai Bian, Jianfeng Chen, Gang Li, Xuejun Wu
• Journal: Biological & pharmaceutical bulletin (Biol Pharm Bull) Vol. 44 Issue 9 Pg. 1254-1262 ( 2021) ISSN: 1347-5215 [Electronic] Japan
• PMID: 34471054 (Publication Type: Journal Article)

Chemical References

• Betacyanins
• Membrane Proteins
• NF-E2-Related Factor 2
• NF-kappa B
• Nfe2l2 protein, mouse
• Tlr4 protein, mouse
• Toll-Like Receptor 4
• betanin
• Heme Oxygenase-1
• Hmox1 protein, mouse
• Pancreatic Elastase

Topics

• Animals
• Aorta, Abdominal (drug effects, pathology)
• Aortic Aneurysm, Abdominal (chemically induced, drug therapy, pathology)
• Betacyanins (pharmacology, therapeutic use)
• Disease Models, Animal
• Heme Oxygenase-1 (metabolism)
• Humans
• Male
• Membrane Proteins (metabolism)
• Mice
• NF-E2-Related Factor 2 (metabolism)
• NF-kappa B (metabolism)
• Pancreatic Elastase (administration & dosage, toxicity)
• Swine
• Toll-Like Receptor 4