Ampelopsin Inhibits Breast Cancer Cell Growth through Mitochondrial Apoptosis Pathway.

Abstract

Ampelopsin, a flavonoid with a wide variety of biological activities, has been proposed to be a potent antitumor agent. However, the mechanism by which Ampelopsin shows anti-breast cancer activity remains unclear. Therefore, this study will explore the mechanism of Ampelopsin's anti-breast cancer activity by culturing MDA-MB-231 and MCF-7 breast cancer cells. Cell Counting Kit-8 (CCK-8) method and plate cloning method were used to detect the proliferation inhibition of breast cancer cells. Fluorescence microscopy was used to detect mitochondrial membrane potential (MMP). 2',7'-Dichlorodihydrofluorescein diacetate (DCFH-DA) method was used to determine the content of intracellular reactive oxygen species (ROS). Hoechst 33258 staining was used to detect the apoptotic morphological changes. Transmission electron microscope was used to observe the mitochondrial structure. Western blot was used to detect the protein expression of Bax and Bcl-2. The results showed that Ampelopsin could significantly inhibit the proliferation of breast cancer cells, and promote cells apoptosis. In addition, the occurrence of apoptosis in breast cancer cells was associated with mitochondrial dysfunction, including the loss of mitochondrial membrane potential, the production of large amounts of reactive oxygen species, and the up-regulation of Bax/Bcl-2 expression. In conclusion, Ampelopsin-induced mitochondria damage leads to loss of mitochondria membrane potential, overproduction of ROS and activation of Bax, increasing mitochondria membrane permeability and ultimately inducing breast cell apoptosis. These findings provided a new perspective on the role of Ampelopsin in breast cancer prevention and treatment.

• Authors: Yue Li, Yong Zhou, Miaoran Wang, Xiaojing Lin, Yunqi Zhang, Irakoze Laurent, Ying Zhong, Jibin Li
• Journal: Biological & pharmaceutical bulletin (Biol Pharm Bull) Vol. 44 Issue 11 Pg. 1738-1745 (Nov 01 2021) ISSN: 1347-5215 [Electronic] Japan
• PMID: 34470980 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents, Phytogenic
• BAX protein, human
• BCL2 protein, human
• Flavonoids
• Plant Extracts
• Proto-Oncogene Proteins c-bcl-2
• Reactive Oxygen Species
• bcl-2-Associated X Protein
• ampelopsin

Topics

• Antineoplastic Agents, Phytogenic (pharmacology, therapeutic use)
• Apoptosis
• Breast Neoplasms (drug therapy, metabolism)
• Cell Proliferation
• Female
• Flavonoids (pharmacology, therapeutic use)
• Humans
• MCF-7 Cells
• Membrane Potential, Mitochondrial (drug effects)
• Mitochondria (drug effects)
• Permeability
• Phytotherapy
• Plant Extracts (pharmacology, therapeutic use)
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• Reactive Oxygen Species (metabolism)
• Vitaceae (chemistry)
• bcl-2-Associated X Protein (metabolism)