Wound management is a major global issue, and there is a growing challenge to develop more effective
hemostatic dressings to control
bleeding and prevent pathogen
infections. In this study, a multifunctional
wound dressing was developed to meet the clinical need. The
hemostatic layer of
wound dressing can quickly stop the
bleeding. Meanwhile, the detection layer is used for real-time fluorescence monitoring of the bacterial colonization. When
infection occurs,
wound dressing is further subjected to illumination for in-situ photodynamic antibacterial treatment. In the rabbit ear artery hemostasis model, the hemostasis time of the
wound dressing was 1 s. The detection limit of the
wound dressing was 1.4 × 105 CFU/cm2 for Escherichia coli, 5.9 × 105 CFU/cm2 for Staphylococcus aureus, and 3.8 × 106 CFU/cm2 for Pseudomonas aeruginosa, respectively. Compared with the control group, an enhanced
wound closure (up to 97.3%) were observed in mice treated with the
wound dressing. In vitro and in vivo experiment results suggested that the
wound dressing was effective in killing pathogenic bacterial and exhibited good biological compatibility, and induced no inflammatory reaction. The proposed design prevents massive
bleeding and
wound infection, and further promotes wound healing. STATEMENT OF SIGNIFICANCE: In this work, we developed a multifunctional
wound dressing, capable of rapid hemostasis, colorimetric monitoring of
bacterial infection, and in situ photodynamic antibacterial. The
hemostatic layer can quickly stop the
bleeding due to its large specific surface area and adsorption pore size for platelet at
bleeding site. Meanwhile, the detection layer can intelligently monitor the
bacterial infection and respond to report
bacterial infection by emitting fluorescence. When
infection occurs,
wound dressing can be used for in-situ photodynamic antibacterial treatment. In vitro and in vivo results showed that the
wound dressing was biocompatible, prevented massive
bleeding and
wound infection, and further promoted wound healing.