Myopia (short-sightedness), usually caused by excessive elongation of the eye during development, has reached epidemic proportions worldwide. In animal systems including the chicken model, several treatments have been shown to inhibit ocular elongation and experimental
myopia. Although diverse in their apparent mechanism of action, each one leads to a reduction in the rate of ocular growth. We hypothesize that a defined set of
retinal molecular changes may underlie growth inhibition, irrespective of the treatment agent used. Accordingly, across five well-established but diverse methods of inhibiting
myopia, significant overlap is seen in the
retinal transcriptome profile (transcript levels and alternative splicing events) in chicks when analyzed by
RNA-seq. Within the two major pathway networks enriched during growth inhibition, that of cell signaling and circadian entrainment,
transcription factors form the largest functional grouping. Importantly, a large percentage of those genes forming the defined
retinal response are downstream targets of the
transcription factor EGR1 which itself shows a universal response to all five growth-inhibitory treatments. This supports EGR1's previously implicated role in ocular growth regulation. Finally, by contrasting our data with human linkage and GWAS studies on
refractive error, we confirm the applicability of our study to the human condition. Together, these findings suggest that a universal set of transcriptome changes, which sit within a well-defined
retinal network that cannot be bypassed, is fundamental to growth regulation, thus paving a way for designing novel targets for
myopia therapies.