Abstract | BACKGROUND/AIMS: METHODS: Four pancreatic cancer cell lines were used for in vitro studies. C57BL/6 mice bearing pancreatic cancer cell-derived xenografts were generated for in vivo studies regarding the therapeutic effects of IR700DX-6T-PDT and IR700DX-mbc94-PDT on pancreatic cancer. The immunostimulatory or immunosuppressive effects of IR700DX-6T-PDT and IR700DX-mbc94-PDT were examined by detecting CD8+ T cells, regulatory T cells (Tregs), and dendritic cells (DCs) using flow cytometry and immunohistochemistry (IHC). RESULTS: TSPO and CB2R were markedly upregulated in pancreatic cancer cells and tissues. Both IR700DX-6T-PDT and IR700DX-mbc94-PDT significantly inhibited pancreatic cancer cell growth in a dose- and time-dependent manner. Notably, assessment of anticancer immune responses revealed that both IR700DX-6T-PDT and IR700DX-mbc94-PDT significantly induced CD8+ T cells, promoted maturation of DCs, and suppressed Tregs, with stronger effects exerted by IR700DX-6T-PDT compared to IR700DX-mbc94-PDT. CONCLUSIONS: IR700DX-6T-PDT and IR700DX-mbc94-PDT involves eliciting anticancer immune responses. Our study has also implicated that PDT in combination with immunotherapy holds promise to improve therapeutic efficacy for patients with pancreatic cancer.
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Authors | Dawei Zhang, Qing Xie, Yang Liu, Zongyan Li, Haiyan Li, Shiying Li, Zhen Li, Jing Cui, Meng Su, Xiaofeng Jiang, Ping Xue, Mingfeng Bai |
Journal | Pharmacological research
(Pharmacol Res)
Vol. 172
Pg. 105811
(10 2021)
ISSN: 1096-1186 [Electronic] Netherlands |
PMID | 34390852
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved. |
Chemical References |
- Bzrp protein, mouse
- IR700DX-mbc94
- Indoles
- Organosilicon Compounds
- Photosensitizing Agents
- Receptor, Cannabinoid, CB2
- Receptors, GABA
- Adenosine Triphosphate
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Topics |
- Adenosine Triphosphate
(metabolism)
- Animals
- CD8-Positive T-Lymphocytes
(drug effects, immunology)
- Cell Line, Tumor
- Cell Survival
(drug effects)
- Dendritic Cells
(drug effects, immunology)
- Humans
- Indoles
(pharmacology, therapeutic use)
- Lymph Nodes
(cytology, immunology)
- Mice, Inbred C57BL
- Organosilicon Compounds
(pharmacology, therapeutic use)
- Pancreas
(metabolism)
- Pancreatic Neoplasms
(drug therapy, immunology, metabolism)
- Photochemotherapy
- Photosensitizing Agents
(pharmacology, therapeutic use)
- Receptor, Cannabinoid, CB2
(metabolism)
- Receptors, GABA
(metabolism)
- Spleen
(cytology, immunology)
- T-Lymphocytes, Regulatory
(drug effects, immunology)
- Mice
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