Scutellarein induces apoptosis and inhibits proliferation, migration, and invasion in ovarian cancer via inhibition of EZH2/FOXO1 signaling.

Abstract

Scutellarein, a flavone found in the perennial herb Scutellaria baicalensis, has antitumorigenic activity in multiple human cancers. However, whether scutellarein can attenuate ovarian cancer (OC) is unclear. This study investigated the effects of scutellarein in OC. In vitro cell viability was assessed using MTT assay whereas proliferation was assessed using 5-ethynyl-2'-deoxyuridine and colony formation assays. Cell apoptosis was detected by an Annexin V-fluorescein isothiocyanate/propidium iodide assay. Wound-healing and Transwell assays were used to determine cell migration and invasion. The differential expression of enhancer of zeste homolog 2 (EZH2) and forkhead box protein O1 (FOXO1) was measured by Quantitative real-time PCR and western blot analysis. We found that scutellarein inhibited viability, migration, invasion of A2780 and SKOV-3 cells, and reduced the expression of EZH2 in OC cells. In addition, FOXO1 was downregulated in OC tissues and cells and negatively regulated by EZH2. Also, scutellarein inhibited tumor growth and metastasis in vivo. In conclusion, scutellarein alleviates OC by the regulation of EZH2/FOXO1 signaling.

• Authors: Xiao Lang, Zheng Chen, Xingyu Yang, Qi Yan, Manfei Xu, Wei Liu, Qin He, Yue Zhang, Weiwei Cheng, Wenxia Zhao
• Journal: Journal of biochemical and molecular toxicology (J Biochem Mol Toxicol) Vol. 35 Issue 10 Pg. e22870 (Oct 2021) ISSN: 1099-0461 [Electronic] United States
• PMID: 34350670 (Publication Type: Journal Article)
• Copyright: © 2021 Wiley Periodicals LLC.

Chemical References

• Antineoplastic Agents
• Drugs, Chinese Herbal
• FOXO1 protein, human
• Forkhead Box Protein O1
• Phytochemicals
• Apigenin
• EZH2 protein, human
• Enhancer of Zeste Homolog 2 Protein
• scutellarein

Topics

• Animals
• Antineoplastic Agents (administration & dosage)
• Apigenin (pharmacology)
• Apoptosis (drug effects, genetics)
• Cell Line, Tumor
• Cell Movement (drug effects, genetics)
• Cell Proliferation (drug effects, genetics)
• Cell Survival (drug effects, genetics)
• Drugs, Chinese Herbal (pharmacology)
• Enhancer of Zeste Homolog 2 Protein (genetics, metabolism)
• Female
• Forkhead Box Protein O1 (metabolism)
• Humans
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Ovarian Neoplasms (drug therapy, metabolism, pathology)
• Phytochemicals (pharmacology)
• Phytotherapy (methods)
• Scutellaria baicalensis (chemistry)
• Signal Transduction (drug effects, genetics)
• Transfection
• Treatment Outcome
• Tumor Burden (drug effects)
• Xenograft Model Antitumor Assays