The human melanoma proteome atlas-Defining the molecular pathology.

Abstract

The MM500 study is an initiative to map the protein levels in malignant melanoma tumor samples, focused on in-depth histopathology coupled to proteome characterization. The protein levels and localization were determined for a broad spectrum of diverse, surgically isolated melanoma tumors originating from multiple body locations. More than 15,500 proteoforms were identified by mass spectrometry, from which chromosomal and subcellular localization was annotated within both primary and metastatic melanoma. The data generated by global proteomic experiments covered 72% of the proteins identified in the recently reported high stringency blueprint of the human proteome. This study contributes to the NIH Cancer Moonshot initiative combining detailed histopathological presentation with the molecular characterization for 505 melanoma tumor samples, localized in 26 organs from 232 patients.

• Authors: Lazaro Hiram Betancourt, Jeovanis Gil, Yonghyo Kim, Viktória Doma, Uğur Çakır, Aniel Sanchez, Jimmy Rodriguez Murillo, Magdalena Kuras, Indira Pla Parada, Yutaka Sugihara, Roger Appelqvist, Elisabet Wieslander, Charlotte Welinder, Erika Velasquez, Natália Pinto de Almeida, Nicole Woldmar, Matilda Marko-Varga, Krzysztof Pawłowski, Jonatan Eriksson, Beáta Szeitz, Bo Baldetorp, Christian Ingvar, Håkan Olsson, Lotta Lundgren, Henrik Lindberg, Henriett Oskolas, Boram Lee, Ethan Berge, Marie Sjögren, Carina Eriksson, Dasol Kim, Ho Jeong Kwon, Beatrice Knudsen, Melinda Rezeli, Runyu Hong, Peter Horvatovich, Tasso Miliotis, Toshihide Nishimura, Harubumi Kato, Erik Steinfelder, Madalina Oppermann, Ken Miller, Francesco Florindi, Qimin Zhou, Gilberto B Domont, Luciana Pizzatti, Fábio C S Nogueira, Peter Horvath, Leticia Szadai, József Tímár, Sarolta Kárpáti, A Marcell Szász, Johan Malm, David Fenyö, Henrik Ekedahl, István Balázs Németh, György Marko-Varga
• Journal: Clinical and translational medicine (Clin Transl Med) Vol. 11 Issue 7 Pg. e473 (07 2021) ISSN: 2001-1326 [Electronic] United States
• PMID: 34323403 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2021 The Authors. Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics.

Chemical References

• Proteome

Topics

• Adult
• Aged
• Aged, 80 and over
• Cell Line, Tumor
• Chromatography, High Pressure Liquid
• Female
• Humans
• Male
• Melanoma (metabolism, pathology)
• Middle Aged
• Proteome (analysis)
• Proteomics (methods)
• Skin Neoplasms (metabolism, pathology)
• Tandem Mass Spectrometry
• Young Adult
• Melanoma, Cutaneous Malignant