Albiflorin alleviates cognitive dysfunction in STZ-induced rats.

Abstract

BACKGROUND:

To explore the effect of albiflorin (AL) on streptozotocin (STZ)-induced Alzheimer's disease (AD) in rats.

METHODS:

A mouse model of diabetic encephalopathy was established by intraperitoneal injection of 1%STZ. Step down test and water maze test were used to test the cognitive function of rats. Congo Red Staining was used to detect the distribution of Aβ plaques in the hippocampus of rats. Cytokine levels in serum and hippocampus were measured using ELISA. Serum insulin, oral glucose tolerance (OGTT), serum superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were measured by commercial kits. And the content of Nrf-2/HO-1/HMGB1/NF-kB in the hippocampus of diabetic rats were detected by western blot.

RESULTS AND CONCLUSION:

Compared with the STZ model group, the average escape latency of rats in the AL group in the Morris water maze test was significantly shortened, and the average number of platform crossings and the ratio of distance/total swimming distance in the target quadrant were increased significantly. Staining of tissue sections and ELISA showed a decrease in Aβ plaque density in the hippocampus of rats in the AL group. And serum insulin levels of rats in the ALgroup were significantly reduced and OGTT was improved. In addition, AL could also regulate the Nrf-2/HO-1/HMGB1/NF-kB signal pathway in the hippocampus. Therefore, AL may ameliorate STZ-induced cognitive impairment in rats by regulating oxidative stress and inflammation in the brain.

• Authors: Xiaojun Ma, Min Song, Yushan Yan, Gaofei Ren, Jingwen Hou, Guijun Qin, Wang Wang, Zhizhen Li
• Journal: Aging (Aging (Albany NY)) Vol. 13 Issue 14 Pg. 18287-18297 (07 28 2021) ISSN: 1945-4589 [Electronic] United States
• PMID: 34319254 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bridged-Ring Compounds
• HMGB1 Protein
• NF-kappa B
• albiflorin
• Malondialdehyde
• Streptozocin

Topics

• Animals
• Bridged-Ring Compounds (pharmacology)
• Cognitive Dysfunction (drug therapy, etiology, pathology)
• Diabetes Mellitus, Experimental (complications, metabolism)
• HMGB1 Protein (metabolism)
• Hippocampus (drug effects, metabolism)
• Male
• Malondialdehyde (metabolism)
• Maze Learning (drug effects)
• NF-kappa B (metabolism)
• Oxidative Stress (drug effects)
• Plaque, Amyloid (pathology)
• Rats
• Rats, Wistar
• Signal Transduction (drug effects)
• Streptozocin (toxicity)