Proteomic analyses identify intracellular targets for Japanese encephalitis virus nonstructural protein 1 (NS1).
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| Abstract |
Japanese encephalitis is a zoonotic disease caused by Japanese encephalitis virus (JEV). JEV nonstructural protein 1 (NS1) is involved in many crucial biological events during viral infection and immune suppression. To investigate the role of JEV NS1 in virus-infected cells, the molecules with which it interacts intracellularly were screened with a pull-down assay and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The interaction between heterogeneous nuclear ribonucleoprotein K (hnRNP K), vimentin and NS1 were verified with coimmunoprecipitation and confocal assays. Our results show that JEV NS1 interacts with vimentin, hnRNP K and colocalizes with cellular vimentin and hnRNP K. Furthermore, our results demonstrate that the expression of vimentin and hnRNP K were up-regulated in both NS1-transfected and JEV-infected cells. Knocking down vimentin and hnRNP K reduced viral replication while conversely, over-expression of vimentin and hnRNP K improved viral replication, suggesting an important role for this protein in the viral life cycle. Also, We found that vimentin also interacted with hnRNP K after overexpression of NS1 or JEV infection. These findings provide insight into the molecular mechanism of JEV replication and highlight the key role the NS1 in JEV infection.
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| Authors | Peng Wang, Xinze Liu, Qi Li, Jue Wang, Wenke Ruan |
| Journal | Virus research (Virus Res) Vol. 302 Pg. 198495 (09 2021) ISSN: 1872-7492 [Electronic] Netherlands |
| PMID | 34175344 (Publication Type: Journal Article) |
| Copyright | Copyright © 2021. Published by Elsevier B.V. |
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