Long non-coding RNA HCP5 functions as a sponge of miR-29b-3p and promotes cell growth and metastasis in hepatocellular carcinoma through upregulating DNMT3A.

Abstract

Multiple studies have revealed that long non-coding RNA (lncRNAs) served as regulatory factors in modulating tumorigenesis of hepatocellular carcinoma (HCC). In the present study, we demonstrated that lncRNA HCP5 was overexpressed in HCC tissues and cell lines, and these findings were obvious even in metastatic and recurrent cases. Knockdown of HCP5 significantly alleviated cell growth, metastasis, and invasion both in vitro and in vivo through promoting apoptosis and by inactivating the epithelial-mesenchymal transition (EMT) progress. Moreover, miR-29b-3p has been identified as a negatively regulatory target gene of HCP5, and served as a tumor suppressor of HCC to prevent cell proliferation, migration, and invasion. Subsequently, DNMT3A was identified as a downstream regulatory factor of miR-29b-3p, and acted as a participated element of HCC progression by activating AKT phosphorylation. Taken together, our study elucidated for the first time that HCP5 plays a crucial role in HCC via the HCP5/miR-29b-3p/DNMT3A/AKT axis and our findings demonstrated a novel diagnostic and therapeutic strategy with potentiality to treat HCC.

• Authors: Yongping Zhou, Kuan Li, Tu Dai, Hong Wang, Zhiyuan Hua, Wuyang Bian, Hao Wang, Fangming Chen, Xiaoming Ai
• Journal: Aging (Aging (Albany NY)) Vol. 13 Issue 12 Pg. 16267-16286 (06 18 2021) ISSN: 1945-4589 [Electronic] United States
• PMID: 34148029 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNMT3A protein, human
• HCP5 long noncoding RNA, human
• MIRN29a microRNA, human
• MicroRNAs
• RNA, Long Noncoding
• DNA (Cytosine-5-)-Methyltransferases
• DNA Methyltransferase 3A
• Proto-Oncogene Proteins c-akt

Topics

• Aged
• Apoptosis (genetics)
• Base Sequence
• Carcinoma, Hepatocellular (genetics, pathology)
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• DNA (Cytosine-5-)-Methyltransferases (genetics, metabolism)
• DNA Methyltransferase 3A
• Disease Progression
• Epithelial-Mesenchymal Transition (genetics)
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Liver Neoplasms (genetics, pathology)
• Male
• MicroRNAs (genetics, metabolism)
• Middle Aged
• Neoplasm Invasiveness
• Neoplasm Metastasis
• Phosphorylation
• Proto-Oncogene Proteins c-akt (metabolism)
• RNA, Long Noncoding (genetics, metabolism)
• Up-Regulation (genetics)