Long non-coding RNA OIP5-AS1 inhibition upregulates microRNA-129-5p to repress resistance to temozolomide in glioblastoma cells via downregulating IGF2BP2.

Abstract

OBJECTIVE:

Long non-coding RNAs (lncRNAs) and miRNAs (miRNAs) participate in tumors, while the effects of lncRNA OIP5 antisense RNA 1 (OIP5-AS1) and miR-129-5p on glioblastoma (GBM) remain to be further studied. We aim to explore the role of OIP5-AS1/miR-129-5p/insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) axis in GBM progression.

METHODS:

OIP5-AS1, miR-129-5p and IGF2BP2 expression in tissues was determined. Temozolomide (TMZ)-resistant GBM cells were established and transfected with relative plasmid to alter OIP5-AS1, IGF2BP2 or miR-129-5p expression. Then, the viability, proliferation, apoptosis and in vivo tumor growth were assessed. The subcellular localization of OIP5-AS1 was determined, and the binding relationships between OIP5-AS1 and miR-129-5p, and between miR-129-5p and IGF2BP2 were confirmed.

RESULTS:

OIP5-AS1 and IGF2BP2 were upregulated whereas miR-129-5p was downregulated in GBM. OIP5-AS1 silencing or miR-129-5p overexpression inhibited GBM cell chemoresistance to TMZ and proliferation, and promoted cell apoptosis. MiR-129-5p downregulation or IGF2BP2 upregulation reversed the role of OIP5-AS1 silencing on GBM cells. OIP5-AS1 sponged miR-129-5p and miR-129-5p targeted IGF2BP2.

CONCLUSION:

OIP5-AS1 inhibition upregulated miR-129-5p to repress resistance to TMZ in GBM cells via downregulating IGF2BP2.

• Authors: Xuan Wang, Xudong Li, Yan Zhou, Xing Huang, Xiaobing Jiang
• Journal: Cell biology and toxicology (Cell Biol Toxicol) Vol. 38 Issue 6 Pg. 963-977 (12 2022) ISSN: 1573-6822 [Electronic] Switzerland
• PMID: 34132932 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2021. The Author(s), under exclusive licence to Springer Nature B.V.

Chemical References

• RNA, Long Noncoding
• MicroRNAs
• Temozolomide
• IGF2BP2 protein, human
• RNA-Binding Proteins
• Mirn129 microRNA, human

Topics

• Humans
• RNA, Long Noncoding (genetics, metabolism)
• MicroRNAs (genetics, metabolism)
• Temozolomide (pharmacology)
• Glioblastoma (drug therapy, genetics)
• Gene Expression Regulation, Neoplastic (genetics)
• Cell Line, Tumor
• Cell Proliferation (genetics)
• RNA-Binding Proteins (genetics, metabolism)