Long non-coding RNA CYTOR regulates proliferation and metastasis of colon cancer cells through regulating miRNA-105/PTEN axis.

Abstract

Colon cancer is a common malignancy, and its incidence and mortality have been increasing in recent years. This study aims to explore the regulation of long non-coding RNA CYTOR on proliferation and metastasis of colon cancer cells through miRNA-105/PTEN axis. Real-time quantitative PCR (qRT-PCR) disclosed that expression of CYTOR was significantly decreased in colon cancer tissues, compared with that of adjacent normal tissues, while miRNA-105 was significantly increased. Correlation study found that CYTOR was negatively correlated with miR-105. The proliferation, migration, and invasion rates of the LoVo cells with highly expressed CYTOR were significantly slower. miR-105 mimic could suppress the decrease in proliferation, migration, and invasion rates of colon cancer cells caused by overexpression of CYTOR. Additionally, the proliferation, migration, and invasion rates of the LoVo cells in miR-105 inhibition group were significantly slower. The Starbase database predicted the targeting of miR-105 by CYTOR, and qRT-PCR and dual luciferase reporter gene method were used to verify the targeting relationship of CYTOR and miRNA-105/PTEN axis. In conclusion, CYTOR can inhibit the proliferation and metastasis of colon cancer cells through targeted inhibition of the miR-105/PTEN axis.

• Authors: Yi Zhang, Weidong Jin, Dandan Ma, Jun Cao, Tao Fu, Zhaolin Zhang, Yang Zhang
• Journal: International journal of clinical and experimental pathology (Int J Clin Exp Pathol) Vol. 14 Issue 4 Pg. 434-443 ( 2021) ISSN: 1936-2625 [Electronic] United States
• PMID: 33936365 (Publication Type: Journal Article)
• Copyright: IJCEP Copyright © 2021.