Abstract | BACKGROUND: METHODS: The expression of circ-OSBPL2, microRNA (miR)-193a-5p, and bromodomain-containing protein 4 (BRD4) was detected using qRT-PCR and Western blot assays. Cigarette smoke extract (CSE)-induced human bronchial epithelial cells (HBECs) was applied to mimic smoke-related COPD in vitro. Flow cytometric analysis of cell apoptosis and ELISA analysis of interleukins (IL)-6, IL-8, tumor necrosis factor-α (TNF-α) levels were performed. The malondialdehyde (MDA) and superoxide dismutase (SOD) production levels were analyzed according to the kit instructions. The binding interaction between miR-193a-5p and circ-OSBPL2 or BRD4 was confirmed by dual- luciferase reporter assay and RNA immunoprecipitation assays. RESULTS: Circ-OSBPL2 was highly expressed in lung tissues of smokers without or with COPD, particularly in smokers with COPD. Also, the expression of circ-OSBPL2 was dose and time-dependently elevated in CSE-induced HBECs. Circ-OSBPL2 down-regulation in HBECs attenuated CSE-evoked cell proliferation arrest, and cell apoptosis, inflammation and oxidative stress promotion. Mechanistically, circ-OSBPL2 served as a sponge for miR-193a-5p, and miR-193a-5p inhibition reversed the effects of circ-OSBPL2 knockdown on CSE-mediated HBECs. Besides that, miR-193a-5p directly targeted BRD4, and miR-193a-5p re-expression in HBECs abolished CSE-induced HBEC injury, which was reverted by BRD4 up-regulation. Additionally, we also found circ-OSBPL2 could indirectly regulate BRD4 via miR-193a-5p. CONCLUSION: Circ-OSBPL2 contributed to the apoptosis, inflammation, and oxidative stress of HBECs in smoke-related COPD by miR-193a-5p/BRD4 axis, suggesting a novel insight on the pathogenesis of COPD and a potential therapeutic strategy for future clinic intervention in COPD.
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Authors | Caifen Zheng, Yongping Zhang, Yingchun Zhao, Yuanfang Duan, Qianghua Mu, Xinying Wang |
Journal | International journal of chronic obstructive pulmonary disease
(Int J Chron Obstruct Pulmon Dis)
2021
Vol. 16
Pg. 919-931
ISSN: 1178-2005 [Electronic] New Zealand |
PMID | 33854310
(Publication Type: Journal Article)
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Copyright | © 2021 Zheng et al. |
Chemical References |
- BRD4 protein, human
- Cell Cycle Proteins
- MicroRNAs
- Nuclear Proteins
- OSBPL2 protein, human
- RNA, Circular
- Receptors, Steroid
- Transcription Factors
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Topics |
- Cell Cycle Proteins
- Humans
- MicroRNAs
(genetics)
- Nuclear Proteins
(genetics)
- Pulmonary Disease, Chronic Obstructive
(genetics)
- RNA, Circular
- Receptors, Steroid
- Transcription Factors
(genetics)
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