Abstract | INTRODUCTION: Evidence on the biological danger associated with surgical plume is lacking. We examined whether surgical plume, generated by the energy devices ultrasonically activated scalpel (US) or electrocautery (EC) contains virus-related substances. MATERIAL AND METHODS: Experiment 1, ex-vivo model: Tumor mass of a hepatocellular carcinoma line was prepared in a Nod/SCID mouse. Surgical plume generated on the mass by US or EC was collected and detection of HBs gene fragment and antigens ( HBsAg or AFP) was conducted. Experiment 2, clinical specimen: Detection of HBV- DNA and HBsAg was conducted following the collection of surgical plume generated from clinically obtained liver specimens from six HBV-associated hepatocellular carcinoma patients. RESULTS: Experiment 1: HBs gene fragment was detected in the solutions regardless of the device used. HBsAg was detected in US and EC solutions and AFP was also detected in a US solution. Experiment 2: HBV- DNA was detected in both devices, in all three cases whose preoperative serum HBV- DNA was positive. In the other serum-negative cases, HBV- DNA was not detected. While serum HBsAg was positive in five of six cases, it was not detected in any solution. CONCLUSIONS:
DNA fragments or antigens of virus can exist in the surgical plume generated by EC or US.
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Authors | Masashi Hirota, Hidekazu Takahashi, Yasuhiro Miyazaki, Tsuyoshi Takahashi, Yukinori Kurokawa, Makoto Yamasaki, Hidetoshi Eguchi, Yuichiro Doki, Kiyokazu Nakajima |
Journal | Minimally invasive therapy & allied technologies : MITAT : official journal of the Society for Minimally Invasive Therapy
(Minim Invasive Ther Allied Technol)
Vol. 31
Issue 5
Pg. 728-736
(Jun 2022)
ISSN: 1365-2931 [Electronic] England |
PMID | 33853487
(Publication Type: Journal Article)
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Chemical References |
- Aerosols
- DNA, Viral
- Hepatitis B Surface Antigens
- alpha-Fetoproteins
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Topics |
- Aerosols
(analysis)
- Animals
- Carcinoma, Hepatocellular
(surgery)
- DNA, Viral
(isolation & purification)
- Electrocoagulation
- Hepatitis B Surface Antigens
(isolation & purification)
- Hepatitis B virus
(isolation & purification)
- Humans
- Liver Neoplasms
(surgery)
- Mice
- Mice, SCID
- Ultrasonic Surgical Procedures
- alpha-Fetoproteins
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