Recombinant antimicrobial peptide microcin J25 alleviates DSS-induced colitis via regulating intestinal barrier function and modifying gut microbiota.

Abstract	Inflammatory bowel disease (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), is rising constantly all over the world. However, current medical treatments are not universally practical. Microcin J25 (MccJ25), a member of the lasso peptides class, has excellent antimicrobial activity both in vitro and in vivo. Here, we assessed the anti-inflammatory effects of MccJ25 through DSS-induced UC mouse model. MccJ25 significantly ameliorated the UC-associated parameters such as decreased body weight, increased disease activity index (DAI) and shortened colon length. MccJ25 also provides barrier protection by preserving structural integrity and reducing inflammatory infiltrates of colon epithelium. The underlying mechanism may be associated with gut microbiota. To test this uncertainty, co-housing experiment was performed, and results indicate homogenized microbiota could relief the inflammatory. Meanwhile, we also proved the prominent role of the possible targets of MccJ25, namely genus Lactobacillus, Bacteroides and Akkermansia (as well as the possible strains related to the important OTUs) in inflammation status through comprehensive analysis. In conclusion, MccJ25 effectively attenuates inflammation and improves disrupted barrier function, and the MccJ25-modified gut microbiota plays a central role in this process.
Authors	Lijun Shang, Haitao Yu, Hongbin Liu, Meixia Chen, Xiangfang Zeng, Shiyan Qiao
Journal	Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (Biomed Pharmacother) Vol. 139 Pg. 111127 (Jul 2021) ISSN: 1950-6007 [Electronic] France
PMID	33819810 (Publication Type: Journal Article)
Copyright	Copyright © 2021 The Authors. Published by Elsevier Masson SAS.. All rights reserved.
Chemical References	Anti-Inflammatory Agents Bacteriocins Pore Forming Cytotoxic Proteins Recombinant Proteins microcin Dextran Sulfate
Topics	Animals Anti-Inflammatory Agents (therapeutic use) Bacteriocins (therapeutic use) Colitis, Ulcerative (chemically induced, drug therapy, microbiology) Colon (enzymology, pathology) Dextran Sulfate Female Gastrointestinal Microbiome (drug effects) Intestinal Mucosa (enzymology, metabolism) Mice Mice, Inbred C57BL Pore Forming Cytotoxic Proteins Recombinant Proteins (therapeutic use)

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