Incorporating redox-sensitive nanogels into bioabsorbable nanofibrous membrane to acquire ROS-balance capacity for skin regeneration.

Abstract

Facing the high incidence of skin diseases, it is urgent to develop functional materials with high bioactivity for wound healing, where reactive oxygen species (ROS) play an important role in the wound healing process mainly via adjustment of immune response and neovasculation. In this study, we developed a kind of bioabsorbable materials with ROS-mediation capacity for skin disease therapy. Firstly, redox-sensitive poly(N-isopropylacrylamide-acrylic acid) (PNA) nanogels were synthesized by radical emulsion polymerization method using a disulfide molecule as crosslinker. The resulting nanogels were then incorporated into the nanofibrous membrane of poly(l-lactic acid) (PLLA) via airbrushing approach to offer bioabsorbable membrane with redox-sensitive ROS-balance capacity. In vitro biological evaluation indicated that the PNA-contained bioabsorbable membrane improved cell adhesion and proliferation compared to the native PLLA membrane. In vivo study using mouse wound skin model demonstrated that PNA-doped nanofibrous membranes could promote the wound healing process, where the disulfide bonds in them were able to adjust the ROS level in the wound skin for mediation of redox potential to achieve higher wound healing efficacy.

• Authors: Shihao Zhang, Yamin Li, Xiaofeng Qiu, Anqi Jiao, Wei Luo, Xiajie Lin, Xiaohui Zhang, Zeren Zhang, Jiachan Hong, Peihao Cai, Yuhong Zhang, Yan Wu, Jie Gao, Changsheng Liu, Yulin Li
• Journal: Bioactive materials (Bioact Mater) Vol. 6 Issue 10 Pg. 3461-3472 (Oct 2021) ISSN: 2452-199X [Electronic] China
• PMID: 33817421 (Publication Type: Journal Article)
• Copyright: © 2021 The Authors.