Rutin prevents inflammation induced by lipopolysaccharide in RAW 264.7 cells via conquering the TLR4-MyD88-TRAF6-NF-κB signalling pathway.

Abstract

OBJECTIVES:

Inflammation widely exists in many diseases and poses a great threat to human and animal health. Rutin, quercetin-3-rhamnosyl glucoside, has a variety of pharmacological effects, including anti-oxidant, anti-inflammatory, antibacterial, anticancer and radioresistance effects. The current study focused on evaluation of its anti-inflammatory activity and described the mechanism of rutin in lipopolysaccharide-induced RAW 264.7 cells.

METHODS:

The related gene and protein expression levels were investigated by quantification real-time PCR and western blotting, respectively.

KEY FINDINGS:

This study revealed that rutin can decrease inducible nitric oxide synthase (iNOS) gene and protein expression levels, effectively increase IκB gene expression, reduce toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), tumour necrosis factor receptor-associated factor 6 (TRAF6) and p65 gene expression and inhibit the phosphorylation of IκB and p65 and the proteins expression of TLR4, MyD88 and TRAF6.

CONCLUSIONS:

These results suggest that rutin might exert anti-inflammatory effect on LPS-stimulated RAW 264.7 cells and will be potentially useful as an adjuvant treatment for inflammatory diseases.

• Authors: Chunlian Tian, Xin Liu, Yu Chang, Ruxia Wang, Mei Yang, Mingchun Liu
• Journal: The Journal of pharmacy and pharmacology (J Pharm Pharmacol) Vol. 73 Issue 1 Pg. 110-117 (Mar 01 2021) ISSN: 2042-7158 [Electronic] England
• PMID: 33791807 (Publication Type: Journal Article)
• Copyright: © The Author(s) 2020. Published by Oxford University Press on behalf of Royal Pharmaceutical Society. All rights reserved. For permissions, please e-mail: [email protected].

Chemical References

• Anti-Inflammatory Agents
• Lipopolysaccharides
• Myd88 protein, mouse
• Myeloid Differentiation Factor 88
• NF-kappa B
• Plant Extracts
• TNF Receptor-Associated Factor 6
• TRAF6 protein, mouse
• Tlr4 protein, mouse
• Toll-Like Receptor 4
• Rutin

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology, therapeutic use)
• Inflammation (chemically induced, prevention & control)
• Lipopolysaccharides
• Macrophages (drug effects, metabolism)
• Mice
• Myeloid Differentiation Factor 88 (metabolism)
• NF-kappa B (metabolism)
• Phosphorylation
• Phytotherapy
• Plant Extracts (pharmacology, therapeutic use)
• RAW 264.7 Cells
• Rutin (pharmacology, therapeutic use)
• Signal Transduction
• TNF Receptor-Associated Factor 6 (metabolism)
• Toll-Like Receptor 4 (metabolism)