Objective: To investigate the Effects of different dusting methods on pathological changes and
cytokines in rat lung tissue. Methods: 84 healthy male SPF Wistar rats, were randomly divided into control group and dynamic dusting group, tracheal perfusion group, On the day 3, 14, 28, and 60 after the dust exposure, 7 rats in each group were randomly selected and killed. Record the weight of rats and calculate the lung coefficient; Observe the pathological changes of lung tissue by HE staining, Evaluate the pulmonary
collagen fibrosis by Masson staining, observe The changes of area ratio of
collagen fibers in lung tissue Sirius scarlet staining, Use
enzyme-linked
immunosorbent assay to detect the content of
transforming growth factor (TGF) -β1 and
hydroxyproline (HYP) in lung tissue homogenate. Results: After the rats were exposed to dust, the dynamic dusting group and the tracheal perfusion group varied significantly in lung histopathology at four time points, and alveolar
inflammation and
Pulmonary fibrosis scores were higher than the control group (P<0.05) ; The percentage of type I and
type III collagen fiber area of tracheal perfusion group was higher than that in the dynamic dusting group in the four time points (P<0.05) ; The content of TGF-β1 in lung tissue homogenate of rats in the tracheal perfusion group was higher than that in the dynamic dusting group on the day 3 and 14 (P<0.05) ; The content of Hyp in the tracheal perfusion group was higher than that in the dynamic dusting group on the day 28 (P<0.05) . Conclusion: The
acute lung injury of rats can be caused by dynamic dusting group and tracheal perfusion group. The lung histopathology shows
inflammation and
fibrosis, but the lung tissue injury of rats is more serious and the pathological changes are more obvious.