Gastric cancer is one of the most common and deadly
cancer types. Currently, four subtypes have been identified with unique molecular alterations: Epstein-Barr virus (EBV)-positive,
microsatellite instability (MSI),
chromosomal instability (CIN), and genomic stable (GS)
tumors. Notably, many gastric
tumors are associated with the bacterium Helicobacter pylori but the genomic landscape of this subgroup of
tumors remains largely unknown. Targeted sequencing covering 425 genes was performed retrospectively on 1703 gastric
tumor tissues and matched normal blood samples. Nonsynonymous mutations, copy-number variation (CNV), and MSI status were called from human
DNA reads; nonhuman
DNA reads were mapped to NCBI microbial reference genome using Kraken and significant species were identified. Overall, 37 (2.76%) from a total of 1703 samples were EBV-positive, 200 (11.74%) samples were H. pylori-positive, and 10 samples were positive for both. Among the rest, 59 (3.46%) samples were MSI, 380 (22.31%) were CIN, and 1017 (59.72%) were GS. Most of the 200 H. pylori-positive samples tend to be genome stable (85.5%, p < 0.001) and microsatellite stable (95%, p = 0.04). Compared to 1017 GS
tumors, mutations in AKT3, EPAS1, MLH1, and BKT and amplifications of NFE2L2,
TERC, MCL1, and TOP1 were significantly enriched in H. pylori-positive
tumors. And compared to EBV-positive
tumors, mutations in PIK3CA, ARID1A, and PTEN were significantly depleted in H. pylori-positive subtype while TP53 mutations were enriched. This study characterized the unique genomic landscape of H. pylori-positive gastric
tumors using targeted panel sequencing. The successful identification of
DNA reads from infectious agents in
tumor samples indicates that deep sequencing is a promising way to uncover characteristics of microbial environment in
tumors.