Gu-Ben-Fang-Xiao decoction modulates lipid metabolism by activating the AMPK pathway in asthma remission.

Abstract

Gu-Ben-Fang-Xiao decoction (GBFXD), derived from the traditional Chinese medicine Yu-Ping-Feng-San, is widely used in clinical settings and has obvious curative effects in respiratory diseases. GBFXD regulates cholesterol transport and lipid metabolism in chronic persistent asthma. There is evidence for its beneficial effects in the remission stage of asthma; however, its metabolic regulatory effects and underlying mechanisms during asthma remission are unclear. In the present study, we used liquid chromatography-mass spectrometry (LC-MS) to analyse the metabolic profile of mouse serum during asthma remission. The acquired LC-MS data were subjected to a multivariate analysis for identification of significantly altered metabolites. In total, 42 metabolites were significantly differentially expressed among the control, model, and GBFXD groups. In particular, levels of fatty acids, acylcarnitines, phosphatidylcholines, phosphatidylethanolamines, phosphatidylinositols, triglycerides, and diacylglycerols were altered during asthma remission. GBFXD may maintain lipid homeostasis on the lung surface by modulating lipid metabolism and may thereby alleviate asthma. We further quantified hypogeic acid (FA 16:1) based on targeted metabolomics and found that GBFXD may regulate fatty acid metabolism by activating the AMP-activated protein kinase (AMPK) pathway. These results support the use of GBFXD in patients with asthma remission.

• Authors: Yan-Nan You, Qiong-Qiong Xing, Xia Zhao, Jian-Jian Ji, Hua Yan, Tao Zhou, Ying-Mei Dong, Li-Shun Ren, Shu-Ting Hou, Yuan-Yuan Ding
• Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (Biomed Pharmacother) Vol. 138 Pg. 111403 (Jun 2021) ISSN: 1950-6007 [Electronic] France
• PMID: 33714782 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 The Authors. Published by Elsevier Masson SAS.. All rights reserved.

Chemical References

• Drugs, Chinese Herbal
• Enzyme Activators
• Gu-Ben-Fang-Xiao decoction
• AMP-Activated Protein Kinases

Topics

• AMP-Activated Protein Kinases (metabolism)
• Animals
• Asthma (drug therapy, metabolism)
• Drugs, Chinese Herbal (pharmacology, therapeutic use)
• Enzyme Activators (pharmacology, therapeutic use)
• Female
• Lipid Metabolism (drug effects, physiology)
• Metabolomics (methods)
• Mice
• Remission Induction (methods)
• Signal Transduction (drug effects, physiology)