Inhibition of lncRNA KCNQ1OT1 Improves Apoptosis and Chemotherapy Drug Response in Small Cell Lung Cancer by TGF-β1 Mediated Epithelial-to-Mesenchymal Transition.

Abstract

PURPOSE:

Drug resistance is one of the main causes of chemotherapy failure in patients with small cell lung cancer (SCLC), and extensive biological studies into chemotherapy drug resistance are required.

MATERIALS AND METHODS:

In this study, we performed lncRNA microarray, in vitro functional assays, in vivo models and cDNA microarray to evaluate the impact of lncRNA in SCLC chemoresistance.

RESULTS:

The results showed that KCNQ1OT1 expression was upregulated in SCLC tissues and was a poor prognostic factor for patients with SCLC. Knockdown of KCNQ1OT1 inhibited cell proliferation, migration, chemoresistance and promoted apoptosis of SCLC cells. Mechanistic investigation showed that KCNQ1OT1 can activate transforming growth factor-β1 mediated epithelial-to-mesenchymal transition in SCLC cells.

CONCLUSION:

Taken together, our study revealed the role of KCNQ1OT1 in the progression and chemoresistance of SCLC, and suggested KCNQ1OT1 as a potential diagnostic and prognostic biomarker in SCLC clinical management.

• Authors: Deyu Li, Qin Tong, Yuane Lian, Zhizhong Chen, Yaru Zhu, Weimei Huang, Yang Wen, Qiongyao Wang, Shumei Liang, Man Li, Jianjing Zheng, Zhenhua Liu, Huanxin Liu, Linlang Guo
• Journal: Cancer research and treatment (Cancer Res Treat) Vol. 53 Issue 4 Pg. 1042-1056 (Oct 2021) ISSN: 2005-9256 [Electronic] Korea (South)
• PMID: 33705625 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• KCNQ1OT1 long non-coding RNA, human
• Potassium Channels, Voltage-Gated
• TGFB1 protein, human
• Transforming Growth Factor beta1

Topics

• Animals
• Antineoplastic Combined Chemotherapy Protocols (therapeutic use)
• Apoptosis
• Biomarkers, Tumor (genetics, metabolism)
• Cell Proliferation
• Drug Resistance, Neoplasm
• Epithelial-Mesenchymal Transition
• Female
• Follow-Up Studies
• Gene Expression Regulation, Neoplastic
• Humans
• Lung Neoplasms (drug therapy, genetics, metabolism, pathology)
• Male
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Middle Aged
• Potassium Channels, Voltage-Gated (genetics)
• Prognosis
• Small Cell Lung Carcinoma (drug therapy, genetics, metabolism, pathology)
• Survival Rate
• Transforming Growth Factor beta1 (genetics, metabolism)
• Tumor Cells, Cultured
• Xenograft Model Antitumor Assays