Gallbladder cancer (GBC) is the most common biliary tract
tumor with a poor prognosis.
Isorhamnetin is a
flavonoid compound extracted from Hippophae rhamnoides L. and has several pharmacological effects including anti-inflammatory and anti-
cancer properties. We treated GBC-SD and NOZ of GBC cell lines with different
isorhamnetin concentrations in vitro. A cell counting kit-8 (CCK-8) assay, transwell assay,
Hoechst 33342 stain assay, flow cytometric analysis, and a colony-forming assay were performed to investigate the effect of
isorhamnetin on the proliferation, apoptosis,
metastasis, and cycle arrest of GBC cells. A western blotting assay was conducted to explore the related
protein expression level of GBC cells. A mice xenograft model and immunohistochemistry staining were employed to assess the effect of
isorhamnetin in vivo.
Isorhamnetin was found to suppress cell proliferation and
metastasis, and trigger apoptosis and arrest the G2/M phase in GBC cells via the inactivation of the PI3K/AKT signaling cascade. Our findings are of clinical significance in providing a novel treatment approach for GBC.