Extraction of high-quality
RNA from pancreatic
tumors for sequencing purposes is technically challenging, as the pancreas is an organ rich in
ribonucleases. The majority of the established
RNA isolation protocols for use with primary pancreatic tissue involve perfusion of
RNA stabilizing
reagent into the pancreatic tissue to protect
RNA integrity before extraction. However, the additional time needed for this procedure can actually lead to further RNA degradation. We optimized a protocol suitable for high quality
RNA isolation from mouse pancreatic
tumors that is a simple, fast, and inexpensive modification of existing methods, combining the use of liquid
nitrogen and
guanidinium thiocyanate-
chloroform extraction. Through this procedure, the mean
RNA Integrity Number value obtained for
RNA isolated from pancreatic
tumors was 9.0, and was reproducibly suitable for RNAseq and qPCR.•a protocol suitable for high quality
RNA isolation from mouse pancreatic
tumors as well as normal pancreas•combining the use of liquid
nitrogen and
guanidinium thiocyanate-
chloroform extraction.