LncRNA TONSL-AS1 participates in coronary artery disease by interacting with miR-197.

Abstract

BACKGROUND:

It has been reported that high expression levels of miR-197 can predict coronary artery disease (CAD). Our bioinformatics analysis showed that miR-197 may bind to long non-coding RNA (lncRNA) TONSL-AS1. This study aimed to investigate the role of TONSL-AS1 in CAD.

METHODS:

This study included 60 CAD patients and 60 healthy controls. Coronary angiography was performed to diagnose CAD. The interaction between TONSL-AS1 and miR-197 was predicted by IntaRNA2.0. Western-blot analysis was performed to illustrate the effect of MTONSL-AS1, miR-197 and BCL2 on human primary coronary artery endothelial cells (HCAECs). Cell migration assay was performed to explore the roles of MTONSL-AS1, miR-197 and BCL2 in regulating cell migration. Cell apoptosis assay was performed to investigate the role of MTONSL-AS1, miR-197 and BCL2 in regulating the apoptosis of HCAECs.

RESULT:

Significant differences in high-density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), and gensini score were observed in patients with CAD. In addition, TONSL-AS1 was downregulated in CAD. Follow-up study revealed that low expression levels of TONSL-AS1 and high expression levels of miR-197 predicted poor survival of CAD patients. Overexpression experiments showed that TONSL-AS1 and miR-197 had no significant effect on the expression of each other. We speculated that MAFG-AS1 may sponge miR-145. Moreover, overexpression of TONSL-AS1 increased, while overexpression of miR-197 decreased the expression levels of BCL2. Furthermore, overexpression of TONSL-AS1 attenuated the effects of overexpression of miR-197 on migration and apoptosis of HCAECs.

CONCLUSIONS:

Therefore, the expression of TONSL-AS1 predicted the survival of CAD patients and it sponged miR-197 to inhibit the apoptosis of HCAECs.

• Authors: Liu Wu, Gang Tan, Xuyong Li, Xiaoli Jiang, Bing Run, Wei Zhou, Hua Liao
• Journal: Microvascular research (Microvasc Res) Vol. 136 Pg. 104152 (07 2021) ISSN: 1095-9319 [Electronic] United States
• PMID: 33662410 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2021 Elsevier Inc. All rights reserved.

Chemical References

• BCL2 protein, human
• Cholesterol, HDL
• Cholesterol, LDL
• MIRN197 microRNA, human
• MicroRNAs
• Proto-Oncogene Proteins c-bcl-2
• RNA, Long Noncoding

Topics

• Adult
• Aged
• Apoptosis
• Case-Control Studies
• Cell Movement
• Cells, Cultured
• Cholesterol, HDL (blood)
• Cholesterol, LDL (blood)
• Coronary Angiography
• Coronary Artery Disease (diagnostic imaging, genetics, metabolism, pathology)
• Coronary Vessels (metabolism, pathology)
• Endothelial Cells (metabolism, pathology)
• Female
• Humans
• Male
• MicroRNAs (genetics, metabolism)
• Middle Aged
• Proto-Oncogene Proteins c-bcl-2 (genetics, metabolism)
• RNA, Long Noncoding (genetics, metabolism)