Abstract | BACKGROUND: METHODS: Bacteria were grown in medium supplemented with 20, 30 and 40 mg/mL of prebiotics inulin or galacto- oligosaccharide, and with or without 5 μM, 25 μM and 125 μM of ferrous sulfate. Colibactin expression was assessed by luciferase reporter assay for the clbA gene, essential for colibactin production, in E. coli Nissle 1917 and by RT-PCR in E. coli NC101. The human epithelial colorectal adenocarcinoma cell line, Caco-2, was used to assess colibactin-induced megalocytosis by methylene blue binding assay and genotoxicity by γ-H2AX immunofluorescence analysis. RESULTS:
Inulin and galacto- oligosaccharide enhanced the expression of clbA in pks+ E. coli. However, the addition of 125 μM of ferrous sulfate inhibited the expression of clbA triggered by oligosaccharides. In the presence of either oligosaccharide, E. coli NC101 increased dysplasia and DNA double-strand breaks in Caco-2 cells compared to untreated cells. CONCLUSION:
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Authors | Manon Oliero, Annie Calvé, Gabriela Fragoso, Thibault Cuisiniere, Roy Hajjar, Ulrich Dobrindt, Manuela M Santos |
Journal | BMC cancer
(BMC Cancer)
Vol. 21
Issue 1
Pg. 172
(Feb 17 2021)
ISSN: 1471-2407 [Electronic] England |
PMID | 33596864
(Publication Type: Journal Article)
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Chemical References |
- Mutagens
- Oligosaccharides
- Peptides
- Polyketides
- colibactin
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Topics |
- Caco-2 Cells
- Carcinogenesis
(chemically induced, pathology)
- Cellular Senescence
- Colonic Neoplasms
(chemically induced, genetics, pathology)
- DNA Damage
- Escherichia coli
(metabolism)
- Genomic Islands
- Humans
- Mutagens
(adverse effects)
- Oligosaccharides
(pharmacology)
- Peptides
(adverse effects)
- Polyketides
(adverse effects)
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