Characterization of a novel polysaccharide from Moutan Cortex and its ameliorative effect on AGEs-induced diabetic nephropathy.
|
| Abstract |
This study aimed to investigate the structure of a new heteropolysaccharide (MC-Pa) from Moutan Cortex (MC), and its protection on diabetic nephropathy (DN). The MC-Pa composed of D-glucose and L-arabinose (3.31:2.25) was characterized with homogeneous molecular weight of 1.64 × 105 Da, and the backbone was 4)-α-D-Glcp-(1 → 5-α-L-Araf-(1 → 3,5-α-L-Araf-(1→, branched partially at O-3 with α-L-Araf-(1 → residue with methylated-GC-MS and NMR. Furthermore, MC-Pa possessed strong antioxidant activity in vitro and inhibited the production of ROS caused by AGEs. In vivo, MC-Pa could alleviate mesangial expansion and tubulointerstitial fibrosis of DN rats in histopathology and MC-Pa could decrease significantly the serum levels of AGEs and RAGE. Western blot and immunohistochemical analysis showed that MC-Pa can reduce the expression of main protein (FN and Col IV) of extracellular-matrix, down-regulate the production of inflammatory factors (ICAM-1 and VCAM-1), and therefore regulate the pathway of TGF-β1. The above indicated that MC-Pa has an improving effect on DN.
|
|---|---|
| Authors | Yuanpei Lian, Maomao Zhu, Juan Chen, Bing Yang, Qinglin Lv, Long Wang, Shuchen Guo, Xiaobin Tan, Chang Li, Weiquan Bu, Wenbo Ding, Xiaobin Jia, Liang Feng |
| Journal | International journal of biological macromolecules (Int J Biol Macromol) Vol. 176 Pg. 589-600 (Apr 15 2021) ISSN: 1879-0003 [Electronic] Netherlands |
| PMID | 33581205 (Publication Type: Journal Article) |
| Copyright | Copyright © 2021. Published by Elsevier B.V. |
| Chemical References |
|
| Topics |
|
Join CureHunter, for free Research Interface BASIC access!
Realize the full power of the drug-disease research graph!