The hypothesis of rescuing
FKBP12/
RyR1 interaction and intracellular
calcium homeostasis through molecular "reshaping" of
FKBP12 was investigated. To this end, novel 4-arylthioalkyl-1-carboxyalkyl-1,2,3-triazoles were designed and synthesized, and their efficacy was tested in human myotubes. A library of 17 compounds (10a-n) designed to dock the
FKBP12/
RyR1 hot-spot interface contact residues, was readily prepared from free α-
amino acids and arylthioalkynes using CuAAC "click" protocols amenable to one-pot transformations in high overall yields and total configurational integrity. To model nitro-oxidative stress, human myotubes were treated with the
peroxynitrite donor SIN1, and evidence was found that some
triazoles 10 were able to normalize
calcium levels, as well as
FKBP12/
RyR1 interaction. For example, compound 10 b at 150 nM rescued 46% of
FKBP12/
RyR1 interaction and up to 70% of resting cytosolic
calcium levels in human myotubes under nitro-oxidative stress. All compounds 10 analyzed showed target engagement to
FKBP12 and low levels of cytotoxicity in vitro. Compounds 10b, 10c, 10h, and 10iR were identified as potential therapeutic candidates to protect myotubes in
muscle disorders with underlying nitro-oxidative stress,
FKBP12/
RyR1 dysfunction and
calcium dysregulation.