In the present study, the concentration of
decorin in canine normal and neoplastic mammary gland tissues was examined to understand the potential role of
decorin in development and progression of canine mammary tumours. The homogenates of 48 mammary gland tumours (10 benign and 38 malignant) and 10 samples of normal canine mammary gland tissue were used in the study. The presence and quantification of
decorin was examined in the homogenates using Western blot and specific canine ELISA. Western blotting confirmed the presence of
decorin both in the normal mammary gland tissues and in the mammary gland tumours. The concentration of
decorin was significantly higher (p < .05) in the benign tumours and non-metastatic malignant tumours than in the normal mammary gland. The concentration of
decorin was significantly lower (p < .05) in the malignant tumours with
metastasis to regional lymph nodes compared with benign tumours and non-metastatic malignant tumours. No significant differences were found in the level of
decorin between the benign and the non-metastatic malignant tumours. Both the histological type of malignant tumours and the histological grade did not significantly affect the concentration of
decorin. These findings suggest that neoplastic transformation in the canine mammary gland leads to increase in the
decorin protein synthesis. The reducing
decorin concentration in canine malignant mammary tumours appears to facilitate the metastatic spread of these tumours.