Key microRNAs and hub genes associated with poor prognosis in lung adenocarcinoma.

Abstract

In the study, we obtained 36 pairs of lung adenocarcinoma (LUAD) tissues and adjacent non-tumorous tissues. Then, we chose a specific hub-target gene of miRNA and used qRT-PCR to evaluate the expression of PECAM1. We found that the expression level of PECAM1 mRNA in LUAD was significantly lower than that in adjacent nontumor tissues (P<0.0001). Univariate and multivariate analyses were conducted on 481 LUAD patients from The Cancer Genome Atlas (TCGA) according to the Cox proportional hazard regression model to evaluate the impact of PECAM1 expression and other clinicopathological factors on survival. The results showed that the low expression of PECAM1 was an important independent predictor of poor overall survival (HR, 0.704; 95% CI, 0.518-0.957; P = 0.025). Based on the Tumor Immune Estimation Resource (TIMER) database, the relationship between PECAM1 expression and B cell, CD8+ T cell, CD4+ T cell, macrophage, neutrophil, and dendritic cell infiltration was weak in LUAD (P<0.01). In particular, a more significant positive correlation between PECAM1 expression and HLA-complex members, CD1C, NRP1, and ITGAX expression in dendritic cell was detected in LUAD. The mechanism which PECAM1 involved in the development of LUAD may be closely related to changes in the immune microenvironment.

• Authors: Guan-Chao Ye, Ya-Fei Liu, Lan Huang, Chun-Yang Zhang, Yin-Liang Sheng, Bin Wu, Lu Han, Chun-Li Wu, Bo Dong, Yu Qi
• Journal: Aging (Aging (Albany NY)) Vol. 13 Issue 3 Pg. 3742-3762 (01 10 2021) ISSN: 1945-4589 [Electronic] United States
• PMID: 33461176 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• MicroRNAs
• PECAM1 protein, human
• Platelet Endothelial Cell Adhesion Molecule-1
• RNA, Messenger

Topics

• Adenocarcinoma of Lung (genetics)
• Gene Regulatory Networks
• Humans
• Kaplan-Meier Estimate
• Lung Neoplasms (genetics)
• MicroRNAs (genetics)
• Multivariate Analysis
• Platelet Endothelial Cell Adhesion Molecule-1 (genetics)
• Prognosis
• Proportional Hazards Models
• Protein Interaction Maps
• RNA, Messenger (metabolism)
• Survival Rate