Abstract | BACKGROUND: Pharmacogenetics is involved in customizing therapy according to the genetic makeup of an individual, and is applicable for chemotherapy, radiotherapy as well as targeted therapy. Drug metabolizing enzymes ( DMEs) involving both phase I, and phase II reactions are widely studied. Our study was involved in whole exome sequencing (WES) of cancer patients, followed by analysis for identifying key variations in DMEs, and associated transporters that have a potential impact on treatment outcome. METHODOLOGY: A total of 181 solid tumor patients at stage >/= III were subjected to WES by the SureSelectXT Human All Exon V6 + UTR library preparation kit, and sequencing in the Illumina NextSeq 550 system. Bioinformatics analysis involved use of GATK pipeline, and the variants were further assessed for population frequency, functional impact with annovar insilico algorithms. Further variant information from significant DMEs, and transporters were extracted and analyzed with PharmGKB to assess level of evidence and infer their impact on the pathways involved in drug response. RESULTS: CONCLUSION: Our analysis detected variations in both phase I and phase II DMEs, as well as associated transporter genes which has been documented to reduce drug efficacy, as well as cause grade 3 and 4 toxicity. Our study reiterates the significance of pharmacogenomics in stratifying patients for appropriate therapy regimen focused at better treatment outcome and quality of life.
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Authors | Mourad A M Aboul-Soud, Alhussain J Alzahrani, Amer Mahmoud |
Journal | Saudi journal of biological sciences
(Saudi J Biol Sci)
Vol. 28
Issue 1
Pg. 628-634
(Jan 2021)
ISSN: 1319-562X [Print] Saudi Arabia |
PMID | 33424349
(Publication Type: Journal Article)
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Copyright | © 2020 The Author(s). |