Abstract | OBJECTIVE: METHODS: Forty male Sprague-Dawley rats were randomly divided into control, chemotherapy, moxibustion and probiotics groups. The IM rat model was established by intraperitoneal injection of 5-Fu. Mild moxibustion treatment and intragastric probiotic administration were provided once daily for 15 days. Tissue morphology, serum levels of inflammatory factors and the expression levels of tight junction proteins, caspase-1, gasdermin D and NLRP3 were evaluated in colon tissue, through hematoxylin and eosin staining, electron microscopy, enzyme-linked immunosorbent assay, Western blotting, quantitative real-time reverse transcription polymerase chain reaction and immunofluorescence. Gut microbiome profiling was conducted through 16S rRNA amplicon sequencing. RESULTS:
Moxibustion and probiotic treatments significantly increased the expression levels of tight junction proteins, reduced cell apoptosis and the expression levels of caspase-1, gasdermin D and NLRP3; they also decreased the serum levels of tumor necrosis factor-α, interleukin (IL)-6, IL-1β and IL-18, while increasing serum levels of IL-10. Moxibustion and probiotic treatments also corrected the reduction in α-diversity and β-diversity in IM rats, greatly increased the proportion of the dominant bacterial genus Lactobacillus and reduced the abundance of the genera Roseburia and Escherichia in chemotherapy-treated rats to levels observed in healthy animals. We also found that these dominant genera were firmly correlated with the regulation of pyroptosis-associated proteins and inflammatory factors. Finally, moxibustion and probiotic treatments elicited similar effects in regulating intestinal host-microbial homeostasis and the expression of NLRP3 inflammasome-related factors. CONCLUSION:
|
Authors | Bing-Rong Li, Shi-Yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-Hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-Meng Zhang |
Journal | Journal of integrative medicine
(J Integr Med)
Vol. 19
Issue 2
Pg. 144-157
(03 2021)
ISSN: 2095-4964 [Print] Netherlands |
PMID | 33353843
(Publication Type: Journal Article)
|
Copyright | Copyright © 2020. Published by Elsevier B.V. |
Chemical References |
- Inflammasomes
- NLR Family, Pyrin Domain-Containing 3 Protein
- Nlrp3 protein, rat
- RNA, Ribosomal, 16S
- Fluorouracil
|
Topics |
- Animals
- Fluorouracil
- Gastrointestinal Microbiome
- Inflammasomes
- Intestinal Mucosa
- Male
- Moxibustion
- Mucositis
(chemically induced, therapy)
- NLR Family, Pyrin Domain-Containing 3 Protein
- RNA, Ribosomal, 16S
- Rats
- Rats, Sprague-Dawley
|