Recurrent chromosomal imbalances provide selective advantage to human embryonic stem cells under enhanced replicative stress conditions.

Abstract

Human embryonic stem cells (hESCs) and embryonal tumors share a number of common features, including a compromised G1/S checkpoint. Consequently, these rapidly dividing hESCs and cancer cells undergo elevated levels of replicative stress, inducing genomic instability that drives chromosomal imbalances. In this context, it is of interest that long-term in vitro cultured hESCs exhibit a remarkable high incidence of segmental DNA copy number gains, some of which are also highly recurrent in certain malignancies such as 17q gain (17q+). The selective advantage of DNA copy number changes in these cells has been attributed to several underlying processes including enhanced proliferation. We hypothesized that these recurrent chromosomal imbalances become rapidly embedded in the cultured hESCs through a replicative stress driven Darwinian selection process. To this end, we compared the effect of hydroxyurea-induced replicative stress vs normal growth conditions in an equally mixed cell population of isogenic euploid and 17q + hESCs. We could show that 17q + hESCs rapidly overtook normal hESCs. Our data suggest that recurrent chromosomal segmental gains provide a proliferative advantage to hESCs under increased replicative stress, a process that may also explain the highly recurrent nature of certain imbalances in cancer.

• Authors: Liselot M Mus, Stéphane Van Haver, Mina Popovic, Wim Trypsteen, Steve Lefever, Nadja Zeltner, Yudelca Ogando, Eva Z Jacobs, Geertrui Denecker, Ellen Sanders, Christophe Van Neste, Suzanne Vanhauwaert, Bieke Decaesteker, Dieter Deforce, Filip Van Nieuwerburgh, Pieter Mestdagh, Jo Vandesompele, Björn Menten, Katleen De Preter, Lorenz Studer, Björn Heindryckx, Kaat Durinck, Stephen Roberts, Frank Speleman
• Journal: Genes, chromosomes & cancer (Genes Chromosomes Cancer) Vol. 60 Issue 4 Pg. 272-281 (04 2021) ISSN: 1098-2264 [Electronic] United States
• PMID: 33336840 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2020 Wiley Periodicals LLC.

Chemical References

• Hydroxyurea

Topics

• Cell Cycle (genetics)
• Cell Division
• Cell Line, Tumor
• Cell Proliferation
• Chromosome Aberrations
• Chromosomes, Human, Pair 17
• DNA Copy Number Variations
• Human Embryonic Stem Cells (cytology)
• Humans
• Hydroxyurea
• Selection, Genetic
• Stress, Physiological
• Transcriptome