Archaeal hyperthermostable mannitol dehydrogenases: A promising industrial enzymes for d-mannitol synthesis.

Abstract

Recently, the term healthy lifestyle connected to low-calorie diets, although it is not possible to get rid of added sugars as a source of energy, despite the close relation of added sugars to some diseases such as obesity, diabetes, etc. As a result, the sweetener market has flourished, which has led to increased demand for natural sweeteners such as polyols, including d-mannitol. Various methods have been developed to produce d-mannitol to achieve high productivity and low cost. In particular, metabolic engineering for d-mannitol considers one of the most promising approaches for d-mannitol production on the industrial scale. To date, the chemical process is not ideal for large-scale production because of its multistep mechanism involving hydrogenation and high cost. In this review, we highlight and present a comparative evaluation of the biochemical parameters that affecting d-mannitol synthesis from Thermotoga neapolitana and Thermotoga maritima mannitol dehydrogenase (MtDH) as a potential contribution for d-mannitol bio-synthesis. These species were selected because purified mannitol dehydrogenases from both strains have been reported to produce d-mannitol with no sorbitol formation under temperatures (90-120 °C).

• Authors: Marwa Yagoub Farag Koko, Wanmeng Mu, Hinawi Abdo Mustafa Hassanin, Shuang Zhang, Han Lu, Jalaleldeen Khaleel Mohammed, Muhammad Hussain, Qi Baokun, Li Yang
• Journal: Food research international (Ottawa, Ont.) (Food Res Int) Vol. 137 Pg. 109638 (11 2020) ISSN: 1873-7145 [Electronic] Canada
• PMID: 33233217 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Review)
• Copyright: Copyright © 2020 Elsevier Ltd. All rights reserved.

Chemical References

• Sweetening Agents
• Mannitol
• Mannitol Dehydrogenases

Topics

• Archaea (metabolism)
• Carbohydrate Metabolism
• Mannitol
• Mannitol Dehydrogenases (genetics, metabolism)
• Sweetening Agents