Alleviation of carbon tetrachloride-induced hepatocellular damage and oxidative stress in rats by Anabaena oryzae phycocyanin.

Abstract

Phycocyanin isolated from Anabaena biomass was in-vitro assayed for its antioxidant activity against DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] free radical, revealing maximum activities amounting to 77 and 80% at 1,000 µg/ml and SC50 values about 96 and 111 µg/ml, respectively. A biological experiment was conducted, involving 40 male Wistar Albino rats, divided into five groups. Group I received only the basal diet as a normal control, while groups II, III, IV, and V were administrated intraperitoneal (IP) injection of a single dose of CCl4 (50% in corn oil) at 0.5 ml/kg body weight. Subsequently, groups II, III, IV, and V received phycocyanin at 0.0, 25, 50, and 100 mg/kg body weight/day. CCl4 induced considerable increases (p < .05) in the levels of serum ALT, AST, urea and creatinine, total lipid, and triglycerides coupled with significant reductions (p < .05) in serum antioxidant enzymes and some liver histopathological deformations compared to the negative control (group 1). Administration of Anabaena oryzae phycocyanin can counteract these CCl4 -induced changes. PRACTICAL APPLICATIONS: Phycocyanin isolated from Anabaena has beneficial effects such as the antioxidant, antibacterial, anticancer, and hepatoprotective effect. Phycocyanin may play a key role in alleviating oxidative stress, artificially induced by carbon tetrachloride in Albino rats, to ultimately determine its capacity to serve as a natural antioxidant for food and health applications.

• Authors: Ali Osman, Ali Salama, Kamal Emam Mahmoud, Mahmoud Sitohy
• Journal: Journal of food biochemistry (J Food Biochem) Vol. 45 Issue 1 Pg. e13562 (01 2021) ISSN: 1745-4514 [Electronic] United States
• PMID: 33184842 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2020 Wiley Periodicals LLC.

Chemical References

• Plant Extracts
• Phycocyanin
• Carbon Tetrachloride

Topics

• Anabaena
• Animals
• Carbon Tetrachloride (toxicity)
• Carcinoma, Hepatocellular
• Chemical and Drug Induced Liver Injury (drug therapy)
• Liver Neoplasms
• Male
• Oxidative Stress
• Phycocyanin (pharmacology)
• Plant Extracts
• Rats
• Rats, Wistar