cMET promotes metastasis and epithelial-mesenchymal transition in colorectal carcinoma by repressing RKIP.

Abstract

Increasing evidence indicates that c-mesenchymal-epithelial transition factor (cMET) plays an important role in the malignant progression of colorectal cancer (CRC). However, the underlying mechanism is not fully understood. As a metastasis suppressor, raf kinase inhibitory protein (RKIP) loss has been reported in many cancer types. In this study, the expression levels of cMET and RKIP in CRC tissues and cell lines were determined, and their crosstalk and potential biological effects were explored in vitro and in vivo. Our results showed that cMET was inversely correlated with RKIP. Both cMET upregulation and RKIP downregulation indicated poor clinical outcomes. Moreover, the MAPK/ERK signaling pathway was implicated in the regulation of cMET and RKIP. Overexpression of cMET promoted tumor cell epithelial-mesenchymal transition, invasion, migration, and chemoresistance, whereas the effects could be efficiently inhibited by increased RKIP. Notably, small hairpin RNA-mediated cMET knockdown dramatically suppressed cell proliferation, although no RKIP-induced influence on cell growth was observed in CRC. Altogether, cMET overexpression may contribute to tumor progression by inhibiting the antioncogene RKIP, providing preclinical justification for targeting RKIP to treat cMET-induced metastasis of CRC.

• Authors: Siyun Wang, Haiqing Ma, Yan Yan, Yu Chen, Sirui Fu, Junjiang Wang, Ying Wang, Hao Chen, Jianhua Liu
• Journal: Journal of cellular physiology (J Cell Physiol) Vol. 236 Issue 5 Pg. 3963-3978 (05 2021) ISSN: 1097-4652 [Electronic] United States
• PMID: 33151569 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2020 Wiley Periodicals LLC.

Chemical References

• PEBP1 protein, human
• Phosphatidylethanolamine Binding Protein
• Proto-Oncogene Proteins c-met

Topics

• Aged
• Animals
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Colorectal Neoplasms (genetics, metabolism, pathology)
• Down-Regulation (genetics)
• Epithelial-Mesenchymal Transition (genetics)
• Female
• Gene Expression Regulation, Neoplastic
• Gene Knockdown Techniques
• Humans
• Kaplan-Meier Estimate
• Liver Neoplasms (secondary)
• MAP Kinase Signaling System
• Male
• Mice, Inbred BALB C
• Mice, Nude
• Middle Aged
• Neoplasm Invasiveness
• Neoplasm Metastasis
• Phosphatidylethanolamine Binding Protein (metabolism)
• Proto-Oncogene Proteins c-met (metabolism)
• Mice