An 18-gene signature based on glucose metabolism and DNA methylation improves prognostic prediction for urinary bladder cancer.

Abstract

BACKGROUND:

Glucose metabolism and DNA methylation play important roles in cancers. We aimed to identify glucose metabolism-related genes that were DNA methylation associated to establish a prognostic signature of bladder cancer (BLCA).

METHODS:

With BLCA sample transcriptome data from The Cancer Genome Atlas (TCGA) and methylation data from TCGA 450 K microarray, glucose metabolism-related genes associated to prognosis and DNA methylation were identified and a prognostic signature was established. GSEA and WGCNA analysis were performed and two genes, UCHL1 and PYCR1, were selected for functional validations.

RESULTS:

18 target genes were identified and the signature based on them was considered an effective and independent prognostic factor. Several pathways were enriched in the high-risk group by GSEA and three modules of genes were identified by WGCNA. UCHL1 and PYCR1 proliferated proliferation, migration and invasion ability of bladder cancer cells.

CONCLUSIONS:

The 18-gene signature is an independent prognostic factor for bladder cancer patients.

• Authors: Zhuonan Liu, Tianshui Sun, Zhe Zhang, Jianbin Bi, Chuize Kong
• Journal: Genomics (Genomics) Vol. 113 Issue 1 Pt 2 Pg. 896-907 (01 2021) ISSN: 1089-8646 [Electronic] United States
• PMID: 33096258 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2020 Elsevier Inc. All rights reserved.

Chemical References

• Biomarkers, Tumor
• UCHL1 protein, human
• Pyrroline Carboxylate Reductases
• Ubiquitin Thiolesterase
• Glucose

Topics

• Biomarkers, Tumor (genetics, metabolism)
• Carcinoma (genetics, metabolism, pathology)
• Cell Line, Tumor
• Cell Movement
• Cell Proliferation
• DNA Methylation
• Glucose (metabolism)
• Humans
• Prognosis
• Pyrroline Carboxylate Reductases (genetics, metabolism)
• Transcriptome
• Ubiquitin Thiolesterase (genetics, metabolism)
• Urinary Bladder Neoplasms (genetics, metabolism, pathology)
• delta-1-Pyrroline-5-Carboxylate Reductase