Circular RNA MTO1 inhibits gastric cancer progression by elevating PAWR via sponging miR-199a-3p.

Abstract

The effect of circular RNA MTO1 (circMTO1) signaling on the expression of miR-199a-3p in gastric carcinoma cells, and its effect on proliferation and apoptosis of gastric cancer cells were investigated in this study. RT-qPCR was performed to detect the expression levels of circMTO1 and miR-199a-3p in the cell lines and tissues of gastric cancer. The effect of circMTO1 and miR-199a-3p on the growth and apoptosis of tumor cells was detected by BrdU incorporation and Annexin V/PI staining. Target gene prediction and screening, and luciferase reporter assays were performed to validate downstream interested genes of circMTO1 and miR-199a-3p. The expression levels of miR-199a-3p target gene PAWR (named as PRKC apoptosis WT1 Regulator Protein) was measured by RT-qPCR and Western blotting. Tumor changes in mice were detected by transfecting circMTO1. The expression of circMTO1 was significantly downregulated in the cell lines and tissues of gastric cancer, and low expression levels of circMTO1 were closely associated with poor prognosis. Overexpression of circMTO1 inhibited tumor growth, enhanced apoptosis rate and decreased cell invasion and migration. There was a significant negative relationship between the expression levels of circMTO1 and miR-199a-3p in gastric cancer tissues. Inhibiting miR-199a-3p expression or overexpression of PAWR could decrease the promotive effects of knockdown of circMTO1 on the progression of gastric cancer, and a positive relationship was established between the expression of circMTO1 and PAWR. circMTO1 can regulate the growth of gastric cancer cells by regulating miR-199a-3p/PAWR axis, thus inhibiting the development and progression of gastric cancer. Abbreviation GC: Gastric cancer; circ RNA: Circular RNA; MTO1: mitochondrial translation optimized 1 homolog.

• Authors: Ruifeng Song, Ya Li, Weiwei Hao, Lei Yang, Bing Chen, Yingying Zhao, Binghua Sun, Feng Xu
• Journal: Cell cycle (Georgetown, Tex.) (Cell Cycle) Vol. 19 Issue 22 Pg. 3127-3139 (11 2020) ISSN: 1551-4005 [Electronic] United States
• PMID: 33089757 (Publication Type: Journal Article, Retracted Publication)

Chemical References

• Apoptosis Regulatory Proteins
• MTO1 protein, human
• MicroRNAs
• RNA, Circular
• RNA-Binding Proteins
• mirn199 microRNA, human
• prostate apoptosis response-4 protein

Topics

• Animals
• Apoptosis (genetics)
• Apoptosis Regulatory Proteins (genetics, metabolism)
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Disease Progression
• Down-Regulation (genetics)
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• MicroRNAs (genetics, metabolism)
• Neoplasm Invasiveness (genetics)
• RNA, Circular (genetics, metabolism)
• RNA-Binding Proteins (genetics)
• Signal Transduction (genetics)
• Stomach Neoplasms (metabolism, pathology)
• Transfection
• Tumor Burden (genetics)
• Xenograft Model Antitumor Assays